4,908 results

1. pUASp YFP Rab10 DN

   Plasmid

   #37709

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab10 (Rab10 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   T23N (dominant negative)

   Promoter

   GAL4

   Available since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

2. GFP-Rab30

   Plasmid

   #31743

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Rab30 (Rab30 Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Actin

   Available since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pBY2807

   Plasmid

   #23222

   Depositor

   Ralf Baumeister

   Article

   Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

   Insert

   Gateway-SnAvi
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Feb. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

   Plasmid

   #86456

   Purpose

   Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)

   Depositor

   Gregor Bucher

   Insert

   3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUAST HRP Rab35 WT

   Plasmid

   #53498

   Depositor

   Matthew Scott

   Article

   Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

   Insert

   Rab35 (Rab35 Fly)
   

   Use

   Tags

   HRP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pUASP YFP Rab11 WT

   Plasmid

   #37711

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab11 (Rab11 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pUASTattB-MKII::nlsLexADBDo

   Plasmid

   #64718

   Purpose

   generating transgenic TRIC flies

   Depositor

   Liqun Luo

   Article

   Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

   Insert

   MKII::nlsLexADBDo
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

   Plasmid

   #169181

   Purpose

   Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   No purification tag and nsp7-nsp8 fusion protein …

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BacPAK GST-Uch37N

   Plasmid

   #19421

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

   Insert

   Uch37 1-239 (UCHL5 Human)
   

   Use

   Tags

   GST and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

10. UAS-u(XL)Cas9

    Plasmid

    #127386

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XL)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAST

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. exon L-firefly

    Plasmid

    #81006

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Use

    Tags

    firefly luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ADAP1

    Plasmid

    #163905

    Purpose

    Full length mouse ADAP1 for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc-HT-Dmp53KRKR

    Plasmid

    #85699

    Purpose

    expression of Drosophila p53 KRKR mutant in Drosophila cells

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    Dmp53 (p53 Fly)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    K26R K302R

    Promoter

    Ac5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

14. Dll3ICD-HPC4 pBacPAK8-3

    Plasmid

    #17334

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Delta-like 3 (Dll3 Mouse)
    

    Use

    Tags

    HPC4

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pSBL_DK198

    Plasmid

    #156425

    Purpose

    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    aa 42-275

    Promoter

    Polyhedrin

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDSAG

    Plasmid

    #62289

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAGW-dpse-GAL4-DBD

    Plasmid

    #125153

    Purpose

    Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
    

    Use

    Gateway fly expression vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSGE_91_4xUAS_dCP

    Plasmid

    #71169

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    91_4xUAS_dCP
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    DSCP Drosophila synthetic core promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUASP dsRed Rab7 DN

    Plasmid

    #44826

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    T22N (dominant negative)

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac1 Flag Su(Z)2

    Plasmid

    #20202

    Depositor

    Nicole Francis

    Article

    Lo et al Mol Cell Biol. 2009 Jan . 29(2):515-25.

    Insert

    Su(Z)2 (Su(z)2 Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only