77,952 results

1. ABE8.20-NG

   Plasmid

   #226857

   Purpose

   Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8.20-NG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Myc-GFP

   Plasmid

   #83375

   Purpose

   Expression of Myc tagged GFP

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   Myc-GFP
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZL Neo Myr Flag SRPK2

   Plasmid

   #20525

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   SRPK2 (SRPK2 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

4. GFP-BAZ1B

   Plasmid

   #65372

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1B (BAZ1B Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Tox-IRES-eGFP

   Plasmid

   #165427

   Purpose

   Expresses TOX in mammalian cells

   Depositor

   Anjana Rao, Patrick Hogan

   Article

   Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

   Insert

   TOX (Tox Mouse)
   

   Tags

   3xflag 5' of Tox

   Expression

   Mammalian

   Promoter

   5' LTR

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR'-NRSF-CITE-GFP

   Plasmid

   #21310

   Depositor

   Jay Nadeau

   Article

   Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

   Insert

   neuron restrictive silencer factor (Rest Mouse)
   

   Use

   Lentiviral

   Tags

   GFP (bicistronic with CITE/IRES)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Oct. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

   Plasmid

   #168806

   Purpose

   PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   ASCL1-P2A-DLX2-T2A-mCherry
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmBanana-Hyg-CTCF-CtermNES-N1

   Plasmid

   #131851

   Purpose

   Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Tags

   mBanana

   Expression

   Mammalian

   Mutation

   the C-terminus of CTCF is linked to HIV-Rev prote…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. htrpC6-YFP

   Plasmid

   #21084

   Depositor

   Craig Montell

   Article

   Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

   Insert

   TrpC6 (TRPC6 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

    Plasmid

    #156436

    Purpose

    For transient expression of human PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5B (PDS5B Human)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104140

    Purpose

    Expresses TREAT-5'TOP reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/HF-CDC50A

    Plasmid

    #209223

    Purpose

    Mammalian expression of CDC50A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    CDC50A (TMEM30A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139093

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dxCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EB3-GFP

    Plasmid

    #190164

    Purpose

    Expresses EB3-GFP in mammalian cells

    Depositor

    Anna Akhmanova

    Article

    Stepanova et al J Neurosci. 2003 Apr 1;23(7):2655-64.

    Insert

    EB3-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-HA-USP38

    Plasmid

    #22580

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP38 (USP38 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-VprBP

    Plasmid

    #133586

    Purpose

    Expresses human VprBP wildtype in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    Vpr (HIV-1) binding protein (DCAF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wildtype human VprBP (Dcaf1)

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD304 VPR-ZF43

    Plasmid

    #138840

    Purpose

    Constitutive expression of VPR-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Calreticulin-N-16

    Plasmid

    #55006

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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