78,002 results

1. pMXs-mHRI-FLAG-IP

   Plasmid

   #101791

   Purpose

   Retroviral vector encoding C-terminally FLAG tagged mouse HRI

   Depositor

   Seiichi Oyadomari

   Article

   Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

   Insert

   Hri (Eif2ak1 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

   Plasmid

   #162379

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. psPAX2-D64V-NC-MS2

   Plasmid

   #122944

   Purpose

   MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   NC-MCP (MS2 coat protein) fusion protein in Gag
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   D64V

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SacI ML GFP Strand 11 Short

   Plasmid

   #164121

   Purpose

   Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Marisa Brini, Tito Calì

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Short
   

   Expression

   Mammalian

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GPR161-Tango

   Plasmid

   #66334

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR161 (GPR161 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. ptf-Galectin3

   Plasmid

   #64149

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin3 (LGALS3 Human)
   

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-HA-CDK4

   Plasmid

   #172612

   Purpose

   Expresses HA-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV-SB100X[EF1α-copGFP]

   Plasmid

   #218323

   Purpose

   Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   copGFP mammalian expression cassette
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. COX8mls-Flag-APEX2-SOPP3

   Plasmid

   #229075

   Purpose

   Expresses APEX2-SOPP3 in the mitochondrial matrix

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-SLC38A9.1

    Plasmid

    #71869

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

11. PB-SAM-Dest

    Plasmid

    #102563

    Purpose

    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    N/A

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF3311

    Plasmid

    #144787

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB3 (CREB3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171617

    Purpose

    AAV Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ClopHensor

    Plasmid

    #25938

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    ClopHensor
    

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Available Since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-human Igf2bp2-IRES-GFP

    Plasmid

    #91890

    Purpose

    Retroviral expression of human Igfbp2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Igf2bp2 (IGF2BP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-HF1

    Plasmid

    #138556

    Purpose

    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

    Plasmid

    #81253

    Purpose

    Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    TetR and LacI
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGolt-mCherry / pGolt3-mCherry

    Plasmid

    #73297

    Purpose

    Visualization of Dendritic Golgi Satellite

    Depositor

    Michael Kreutz

    Article

    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

    Inserts

    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    

    Tags

    ER export and mCherry

    Expression

    Mammalian

    Promoter

    CMV immediate early promoter

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cyto-V5-APEX2

    Plasmid

    #170572

    Purpose

    Expresses APEX2 in cytosol

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLacO-pEF1α-RFP

    Plasmid

    #179507

    Purpose

    Expresses monomeric RFP in mammalian cells under a EF1α promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    monomeric RFP
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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