Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Mammalian Expression Clear filter

Applied Filters

Mammalian Expression Clear filter

80,047 results

Showing: 8581 - 8600 of 80047 results

  2. pvPE-V4-Puro

    Plasmid
    #240290
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4-Puro
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240290-plasmid-map-sequence-id-488804

    Enlarge

  4. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

    Enlarge

  5. pEGFP-N3-FLuc-5BoxB_O

    Plasmid
    #147079
    Purpose
    Mammalian Expression of 5BoxB
    Depositor
    Elisa Izaurralde
    Insert
    5BoxB
    Expression
    Mammalian
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    147079-plasmid-map-sequence-id-355330

    Enlarge

  7. PB-TAC-ERN

    Plasmid
    #80475
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80475-plasmid-map-sequence-id-477521

    Enlarge

  11. Lenti-LucOS

    Plasmid
    #22777
    Depositor
    Tyler Jacks
    Insert
    OVA-SIY
    Use
    Cre/Lox and Lentiviral
    Tags
    luciferase
    Expression
    Mammalian
    Available Since
    Dec. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22777-plasmid-map-sequence-id-419746

    Enlarge

  12. pEGFP-C1-SopF

    Plasmid
    #137734
    Purpose
    Expresses EGFP fused to SopF
    Depositor
    Leigh Knodler
    Insert
    SopF
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137734-plasmid-map-sequence-id-271547

    Enlarge

  13. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid
    #178320
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi
    Inserts
    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178320-plasmid-map-sequence-id-363290

    Enlarge

  14. TFORF0600

    Plasmid
    #141644
    Purpose
    Lentiviral vector for overexpressing the MZF1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MZF1 (MZF1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141644-plasmid-map-sequence-id-389703

    Enlarge

  16. mApple-Golgi-7

    Plasmid
    #54907
    Purpose
    Localization: Golgi Complex, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. ADORA2B-DuET

    Plasmid
    #213175
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADORA2B (ADORA2B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213175-plasmid-map-sequence-id-430743

    Enlarge

Showing: 8581 - 8600 of 80047 results