78,130 results

1. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

   Plasmid

   #193346

   Purpose

   tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Th-iCre

   Plasmid

   #159459

   Purpose

   Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   P2A-iCre
   

   Use

   Cre/Lox

   Tags

   P2A

   Expression

   Mammalian

   Promoter

   Endogenous TH

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro-C9orf72

   Plasmid

   #83439

   Purpose

   Expresses Cas9 and a gRNA targeting C9orf72

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human C9orf72 (C9orf72 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-RAP1A-WT-puro

   Plasmid

   #156166

   Purpose

   Lentiviral vector for expression of RAP1A-WT, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHLmMBP-1

   Plasmid

   #72343

   Purpose

   Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBITE-EGFRvIII-F263-OKT3

   Plasmid

   #190680

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV JMJD3

   Plasmid

   #21212

   Depositor

   Paul Khavari

   Article

   Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   May 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-CANX

   Plasmid

   #227279

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CANX (CANX Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #161927

   Purpose

   Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   Surface marker and Citrine repression reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pEF

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Venus1-Y705F/S727A-STAT3

    Plasmid

    #123176

    Purpose

    Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    Double Y705F and S727A substitutions

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCiES

    Plasmid

    #60010

    Purpose

    Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Insert

    Env (foamy virus)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-p150

    Plasmid

    #51412

    Purpose

    expression of full length of chicken DCTN1 p150Glued in mammalian cells

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    DCTN1 (DCTN1 Chicken)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pLPCX-PECAMTS

    Plasmid

    #45850

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    PECAM1 (PECAM1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDV05.2_pEF1a-iRFP720_secreted

    Plasmid

    #200409

    Purpose

    Expresses a secreted version of iRFP720 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (secreted)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMN HA-NDP52

    Plasmid

    #199307

    Purpose

    For expression of the mammalian NDP52 tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

    Plasmid

    #112925

    Purpose

    Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6 promoter, gRNA-SAM expression cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-BCL6-IRES-GFP

    Plasmid

    #31391

    Depositor

    Hilda Ye

    Article

    Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

    Insert

    B-cell lymphoma 6 (BCL6 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CamKIIa-jGCaMP8f-WPRE

    Plasmid

    #176750

    Purpose

    AAV-mediated expression of GCaMP8f under the CamKIIa promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CamKIIalpha

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX_314-HRI-V5

    Plasmid

    #202434

    Purpose

    Expression of HRI

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid

    #188072

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560, deletion of aa2-6

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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