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ChrII_ttTi5605_loxP_myo2_neoR

Plasmid

#194056

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

Depositor

Kota Mizumoto

Publication

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ttTi5605

Expression

Worm

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEY26

Plasmid

#191031

Purpose

cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.3-2.6k) (cho-1 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY51

Plasmid

#191054

Purpose

mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mec-3 (mec-3 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY39

Plasmid

#191043

Purpose

acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(2.1k) (acr-2 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY52

Plasmid

#191055

Purpose

odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

odr-1 (odr-1 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY43

Plasmid

#191047

Purpose

eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

eat-4(prom6-1) (eat-4 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Publication

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS791

Plasmid

#188889

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

Depositor

Erik Jorgensen

Publication

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

Plasmid

#182353

Purpose

GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pWZ194

Plasmid

#163640

Purpose

rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Publication

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)

Use

CRISPR

Tags

2xmKate2 and GFP

Expression

Worm

Promoter

rps-27

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2644

Plasmid

#179632

Purpose

Pnmr-1::TetanusToxin-UrSL-wCherry unc-54 3' UTR C.elegans AVA/E/D premoter IN and others expression of TetanusToxin-UrSL-wCherry

Depositor

Mei Zhen

Publication

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TetanusToxin

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJH1579

Plasmid

#178904

Purpose

Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60

Depositor

Mei Zhen

Publication

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

YC3.60

Expression

Worm

Mutation

See Depositor Comments Below

Promoter

Pglr-1

Available since

Jan. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCZGY405

Plasmid

#172694

Purpose

Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

Depositor

Yishi Jin

Publication

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

rab-3 (rab-3 Nematode)

Tags

Punc-25 and mcherry

Expression

Worm

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK136

Plasmid

#173744

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CIBN-3xFLAG::MP::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK138

Plasmid

#173746

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK260

Plasmid

#173753

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TC646

Plasmid

#164892

Purpose

Pdpy30::loxP::mcherry::let-858terminator::loxP::GFP

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

GFP

Expression

Worm

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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TC872

Plasmid

#164895

Purpose

TRE::nCre, inducible nCre expression when co-express with rtTA

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

nCre

Expression

Worm

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Plasmid

#159874

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Plasmid

#159866

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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