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pMB73

Plasmid

#73086

Purpose

Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCM5.32

Plasmid

#19397

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mes-2 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pJW1883

Plasmid

#154331

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBCN42-R4R3

Plasmid

#34917

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Type

Empty backbone

Expression

Worm

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pSR47

Plasmid

#69258

Purpose

pmyo-3::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

myo-3

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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L3785

Plasmid

#1592

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, T7p, gfpN, IVSR, gfpS65C, IVS…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pBN283

Plasmid

#86728

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdat-1::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSAH61 - [ASELp | wrmScarlet | tbb-2 UTR]

Plasmid

#200335

Purpose

mScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Insert

[ASELp | wrmScarlet | tbb-2 UTR]

Expression

Worm

Promoter

WBGene00001534, gcy-7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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L4667

Plasmid

#1669

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, IVSA, rf2128, ATG-MitLS, gfpN…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pCFJ1656

Plasmid

#51483

Purpose

miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pBCN24-R4R3

Plasmid

#26304

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Type

Empty backbone

Expression

Worm

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pCZGY2840

Plasmid

#87764

Purpose

Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pmec-4

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2844

Plasmid

#87760

Purpose

Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Punc-17b

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJW1947

Plasmid

#154339

Purpose

Enhancer reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

Plasmid

#159886

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-1(no_atg)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCM5.48

Plasmid

#19385

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mex-5 3'utr in pDONRP2RP3 (mex-5 Nematode)

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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L2603

Plasmid

#1511

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, ATG-SecSig, pes10

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pNM3734

Plasmid

#154118

Purpose

mec-4 promoter driving GAL4-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::Gal4SK::QFAD::tbb-2 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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