1,748 results

1. pDD291

   Plasmid

   #137019

   Purpose

   mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   mKate2-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159867

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCM5.48

   Plasmid

   #19385

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   mex-5 3'utr in pDONRP2RP3 (mex-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pASH3 [pmyo-3::BeCNG1-YFP]

   Plasmid

   #168167

   Purpose

   Expression of BeCNG1-YFP in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   BeCNG1-YFP
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSM12

   Plasmid

   #139035

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   dendra2 (no STOP codon)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCFJ2215 - Intron GG3 - 900 bp

   Plasmid

   #159882

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG3 - 900 bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV1951

   Plasmid

   #37832

   Depositor

   Gary Silverman

   Article

   Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

   Insert

   Pnhx-2mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   nhx-2

   Available since

   Nov. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pMLS546

   Plasmid

   #188883

   Purpose

   Integrating Pmex-5::Cas9 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Pmex-5::Cas9
   

   Use

   Tags

   Expression

   Worm

   Mutation

   C. elegans codom optimization

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Pmyo3::tag:sl1(G16C)

   Plasmid

   #79003

   Purpose

   Expression of exogenous SL1 in C. elegans muscle

   Depositor

   Xiao Liu, Ge Zhan

   Article

   Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

   Insert

   sls-1.2 (sls-1.2 Nematode)
   

   Use

   Tags

   illumina-adapter

   Expression

   Worm

   Mutation

   G16C

   Promoter

   myo-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L3670

    Plasmid

    #1586

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    let858Tsa, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, …

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXF130

    Plasmid

    #139041

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    mkate2 (includes STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAJ50

    Plasmid

    #14918

    Depositor

    James Lok

    Article

    Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

    Insert

    enhanced monomeric red fluorescent protein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBCN43-R4R3

    Plasmid

    #34918

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGC95

    Plasmid

    #19611

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phsp-16.41::FLP::let-858(3’)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGC146

    Plasmid

    #19613

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phsp-16.2::FLP::let-858(3’)-(Cbr)unc-119(+)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.2

    Available since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pID2.02

    Plasmid

    #26955

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH3666

    Plasmid

    #191447

    Purpose

    Pacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH2930

    Plasmid

    #175283

    Purpose

    Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-16a (daf-16 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pges-1

    Available since

    Dec. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pJH3043

    Plasmid

    #175284

    Purpose

    Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    daf-16a mini-gene (daf-16 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pdpy-30

    Available since

    Nov. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid

    #164266

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge