1,779 results

1. L3670

   Plasmid

   #1586

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   let858Tsa, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, …

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWK61

   Plasmid

   #163642

   Purpose

   rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Nematode, Human)
   

   Use

   CRISPR

   Tags

   2xmKate2 and GFP

   Expression

   Worm

   Promoter

   rps-27

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

   Plasmid

   #164266

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6 promoter from W05B2.8

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW1592

   Plasmid

   #121059

   Purpose

   GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   GFP^SEC^BioTag::AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

   Expression

   Worm

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1850

   Plasmid

   #154328

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1849

   Plasmid

   #154327

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCM5.33

   Plasmid

   #19396

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   fbf-1 3'utr in pDONRP2RP3
   

   Expression

   Worm

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMLS546

   Plasmid

   #188883

   Purpose

   Integrating Pmex-5::Cas9 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Pmex-5::Cas9
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTY1048-S65T-LacI

   Plasmid

   #34853

   Depositor

   Paul Sternberg

   Article

   Gonzalez-Serricchio et al BMC Genet. 2006 Jun 7;7:36.

   Insert

   GFP-LacI
   

   Expression

   Worm

   Mutation

   GFP is mutated: S65T and contains introns, in ord…

   Available Since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

    Plasmid

    #159898

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
    

    Expression

    Worm

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXF222

    Plasmid

    #139032

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    mkate2 (no STOP codon)
    

    Expression

    Worm

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG412

    Plasmid

    #40087

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Type

    Empty backbone

    Tags

    Dendra2 PAFP, S-peptide, and TEV protease cleavag…

    Expression

    Worm

    Promoter

    pie-1

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD162.21

    Plasmid

    #27511

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pmyo-3::dsRed::let-858_3'UTR
    

    Expression

    Worm

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGC76

    Plasmid

    #19641

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <4xNLS-GFP::let-858(3’)
    

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPD95_02

    Plasmid

    #1478

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    T3p, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, T7p, …

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid

    #154838

    Purpose

    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    synthetic guide::∆HYGR::unc-54 3' UTR
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    ∆HYGR is promoterless and encodes aa 60-341

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH3666

    Plasmid

    #191447

    Purpose

    Pacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Tags

    wCherry

    Expression

    Worm

    Promoter

    Pacr-2s

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH3043

    Plasmid

    #175284

    Purpose

    Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    daf-16a mini-gene (daf-16 Nematode)
    

    Expression

    Worm

    Promoter

    Pdpy-30

    Available Since

    Nov. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. L4663

    Plasmid

    #1665

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    myo3-2341, myo3Tsa, SV40NLS, gfpN, IVSR, gfpY66W,…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHA843 - hrp-1p::hrp-1HsLCdeltaLC

    Plasmid

    #139200

    Purpose

    Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Expression

    Worm

    Mutation

    stop at beginning of last exon

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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