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pSR11

Plasmid

#69154

Purpose

read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
fzr-1 (fzr-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

fzr-1 and rps-27

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Use

Tags

GFP(65C)

Expression

Worm

Mutation

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMyo-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68044

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-3::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD95.75 RSF-1

Plasmid

#66863

Purpose

Generate transgenic C..elegans expressing GFP-fused RSF-1

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
AP303-4

Plasmid

#66088

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
AP334-5

Plasmid

#66096

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
APq5271

Plasmid

#66085

Purpose

co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa4-2

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
APCSD 54

Plasmid

#66100

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 54

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
APCSD 53

Plasmid

#66099

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 53

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP334-6

Plasmid

#66097

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP334-2

Plasmid

#66095

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-4

Plasmid

#66094

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-3

Plasmid

#66093

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-2

Plasmid

#66092

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
AP332-4

Plasmid

#66091

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs4

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
AP332-3

Plasmid

#66090

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs4

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
AP332-2

Plasmid

#66089

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs4

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
AP303-3

Plasmid

#66087

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
AP54-5

Plasmid

#65599

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
AP56-6

Plasmid

#65593

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-5

Plasmid

#65591

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-8

Plasmid

#65596

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-7

Plasmid

#65594

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP54-3

Plasmid

#65597

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
pAD-g6

Plasmid

#61497

Purpose

For expression of C. briggsae gld-1, used for rescue of C. elegans q485 homozygotes

Depositor

Eric Haag

Article

Beadell et al Proc Natl Acad Sci U S A. 2011 Dec 6;108

Insert

gld-1 (Cbr-gld-1 Caenorhabditis briggsae)

Use

Tags

FLAG and His

Expression

Worm

Mutation

Promoter

gld1

Available since

Feb. 25, 2015

Availability

Academic Institutions and Nonprofits only
pEL333

Plasmid

#52349

Purpose

swan-1 co-IP construct

Depositor

Erik Lundquist

Article

Yang et al Genetics. 2006 Dec;174(4):1917-32. Epub

Insert

swan-1 (swan-1 Nematode)

Use

Tags

3x FLAG

Expression

Worm

Mutation

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEL481

Plasmid

#52351

Purpose

GFP reporter for rack-1

Depositor

Erik Lundquist

Article

Demarco et al PLoS Genet. 2010 Nov 18;6(11):e1001215.

Insert

rack-1 (rack-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH009

Plasmid

#42146

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP-tag::mTFP1

Use

Tags

TAP-Tag

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNG2470

Plasmid

#37837

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

F13D12.6(G166R) (F13D12.6 Nematode)

Use

Tags

YFP

Expression

Worm

Mutation

G166R, S168G

Promoter

nhx-2

Available since

Jan. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNG2462

Plasmid

#37836

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2F13D12.6::YFP (F13D12.6 Nematode)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

nhx-2

Available since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge