8,086 results
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Plasmid#133469PurposeIntegration into fission yeast genome at lys3 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
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YIplac211-NHX1-HaloTagx2
Plasmid#177107PurposeExpresses Nhx1-HaloTagx2 in yeast cellsDepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-EGFP-ccdB
Plasmid#14312DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP43
Plasmid#65519PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionYeastMutationPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-ccdB-HA
Plasmid#14242DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CDC10
Plasmid#51568PurposeConstitutively express bud neck localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCDC10 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP39
Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastMutationPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ222
Plasmid#52223PurposeConstitutive expression of humanized Cas9DepositorInsertCas9
UseTagsExpressionYeastMutationsilent mutation in the original CspCI sitePromoteradh1Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH728-CEN-RLuc/maxCFLuc
Plasmid#38212DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-Cerulean-ccdB
Plasmid#14418DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUB23-M-beta-gal
Plasmid#8816DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationM (methionine) at amino terminus of beta-galPromoterAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pL-Sapphire
Plasmid#176568PurposeExpression of Sapphire for auxotrophic selection in the absence of leucineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-EGFP-ccdB
Plasmid#14321DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-PRUNE-HA
Plasmid#183945PurposeExpresses human HA-tagged PRUNE from yeast GPD promoterDepositorAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK59-ATG17-GFP
Plasmid#44175DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK036
Plasmid#171308PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertamdS ANIA_08777 acetamidase, selection marker
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-SpHis5
Plasmid#44845PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selectionDepositorInsertyomKO2
UseTagsExpressionYeastMutationPromoterAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMOD6-pGRR-r11-r10-yeGFP
Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorArticleInsertyeGFP
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterpGRR-r11-r10Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Erg11-iGFPx3
Plasmid#115422PurposeExpress Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertErg11-iGFPx3
UseTagsiGFPExpressionYeastMutationPromoterEndogenousAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only