8,058 results

1. pFA6a-link-yomKO2-SpHis5

   Plasmid

   #44845

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. YIp204-PADH1-AFB2

   Plasmid

   #99531

   Purpose

   S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

   Depositor

   Helle Ulrich

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p405MET3

   Plasmid

   #15962

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MET3 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDE731

   Plasmid

   #103008

   Purpose

   S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   Fncpf1
   

   Use

   Tags

   3HA and NLS

   Expression

   Yeast

   Mutation

   human codon optimized

   Promoter

   TEF1

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV0758

   Plasmid

   #133511

   Purpose

   Expression of 3GFP tagged RasAct probe to monitor active Ras1

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV0783

   Plasmid

   #133515

   Purpose

   Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB3034(X-3 MarkerFree)

   Plasmid

   #73272

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPS811

   Plasmid

   #8904

   Depositor

   Pamela Silver

   Article

   Lee et al Genes Dev 1996 May 15;10(10):1233-46.

   Insert

   NPL3 (NPL3 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS35

   Plasmid

   #83797

   Purpose

   Useful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   mCherry
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE HIS3

    Plasmid

    #122877

    Purpose

    HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRFLIP42

    Plasmid

    #65518

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-mCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRFLIP35

    Plasmid

    #65513

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-TFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRFLIP32

    Plasmid

    #65511

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ376

    Plasmid

    #74213

    Purpose

    Combined sgRNA/Cas9 vector with KanMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA CRU HISMX

    Plasmid

    #131164

    Purpose

    PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    CRU

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA NuiHA-loxP-nat-CUP1p

    Plasmid

    #131170

    Purpose

    PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    Nui-HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZ-mouseGIRK2-eGFP-StII-6xHis

    Plasmid

    #124277

    Purpose

    Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Use

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PhyB-mCherry-NLS

    Plasmid

    #51571

    Purpose

    Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    NLS and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. G31, Cdc20-AID tagging

    Plasmid

    #130269

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    

    Use

    Tags

    AID(71-114)

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKmK.C4

    Plasmid

    #125062

    Purpose

    Storage plasmid for K.marxianus ARS

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    KmARS2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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