8,058 results

1. pDRF1-GW ymNeongreen-T2A-mTurquoise2

   Plasmid

   #125701

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mTurquoise
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-CLIP-KanMX6

   Plasmid

   #87025

   Purpose

   Tag S. pombe genes in C terminal with CLIP

   Depositor

   Julien Berro

   Insert

   CLIP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCLUC

   Plasmid

   #1280

   Depositor

   Susan Lindquist

   Article

   Kimura et al Science 1995 Jun 2;268(5215):1362-5.

   Insert

   CUP1-lacZ fusion (CUP1-1 Budding Yeast)
   

   Use

   Tags

   CUP1 promoter

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pMEL12

   Plasmid

   #107918

   Purpose

   hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG303GAL-ccdB-EGFP

   Plasmid

   #14181

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV113

   Plasmid

   #63180

   Purpose

   pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPS1372

   Plasmid

   #1353

   Depositor

   Pamela Silver

   Article

   Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

   Insert

   pADH-NLS-NES-GFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pFA6a-link-yomWasabi-Kan

   Plasmid

   #44945

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomWasabi
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRP2000

   Plasmid

   #159309

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGPD-luxAB(HIS)

    Plasmid

    #1106

    Depositor

    Susan Lindquist

    Article

    Parsell et al Nature 1994 Dec 1;372(6505):475-8.

    Insert

    bacterial luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pKT0140

    Plasmid

    #8732

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECitrine
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with S65G, V68L, Q69M, S72A, T203Y

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. APEX2 VLP

    Plasmid

    #182211

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology; Tem imaging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSWHhc-p53

    Plasmid

    #133903

    Purpose

    Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    p53 (TP53 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GAL 72Q+ProGFPp416

    Plasmid

    #15582

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q + Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pFA6a-link-yomEos2-CaURA3

    Plasmid

    #44887

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEos2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAL-SKI1-NAT

    Plasmid

    #160382

    Purpose

    Overxpression of SKI1/XRN1 in yeast

    Depositor

    John McCusker

    Insert

    SKI1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOP459

    Plasmid

    #85933

    Purpose

    Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 2G4 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 426Gal-FUS-RRM Mutant-YFP

    Plasmid

    #29608

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS RRM mutant (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    F305L, F341L, F359L and F368L

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pFA6a-6xGLY-Myc-HIS3MX6

    Plasmid

    #20768

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge