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pRB2963

Plasmid

#8678

Depositor

David Botstein

Publication

Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

Insert

GFP::TUB1-828 under GAL1 promoter (TUB1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
RMC sup35 promotor

Plasmid

#1174

Depositor

Susan Lindquist

Publication

Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

Insert

Rnq1-Sup35 fusion (SUP35, RNQ1 Budding Yeast)

Expression

Yeast

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pCHFLAGGR

Plasmid

#1279

Depositor

Susan Lindquist

Publication

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GR (Nr3c1 Rat)

Tags

FLAG

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pUSti1

Plasmid

#1335

Depositor

Susan Lindquist

Publication

Chang et al Mol Cell Biol 1997 Jan;17(1):318-25.

Insert

Sti1 (STI1 Budding Yeast)

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
p2HGAL/GR/CYC

Plasmid

#1220

Depositor

Susan Lindquist

Publication

Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

Insert

GR (Nr3c1 Rat)

Use

2micron

Expression

Yeast

Mutation

pRS423 with GAL promoter and CYC terminator, 2mic…

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Gal tr-lux

Plasmid

#1330

Depositor

Susan Lindquist

Publication

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

Bacterial luciferase-V.h.CTP5

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pML104-KanMX-sgRNAv2

Plasmid

#254712

Purpose

Yeast CRISPR-Cas9 backbone with sgRNA cassette for rapid PCR-based guide insertion; KanMX/G418 selection in S. cerevisiae, Amp/Kan propagation in E. coli.

Depositor

Brian Strahl

Publication

Rostamian et al STAR Protoc. 2026 Jul 28;7(3):104751. do

Insert

YKU70 sgRNA (YKU70 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52 promoter

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pHLP785

Plasmid

#253572

Purpose

Integration of OsTIR1(F74A)-3Myc expressed from C. albicans TDH3 promoter with recyclable SAT1 (nourseothricin) selection marker in C. albicans for use in AID2 system.

Depositor

Mark Hall

Insert

OsTIR1(F74A)-3Myc

Expression

Yeast

Mutation

F74A variant of Oryza sativa TIR1 gene

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pRS118

Plasmid

#234878

Purpose

CRISPR-Cas9 plasmid for use in Candida albicans. Contains Cas9, NEUT5L integration site, and the sgRNA cloning site (SNR52 promoter)

Depositor

Rebecca Shapiro

Publication

Després et al Genetics. 2025 Oct 8;231(2):iyaf154. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pRS303H-Padh-OsTIR1(F74G)

Plasmid

#247625

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRS303K-Pgal-OsTIR1(F74G)-GEM

Plasmid

#247622

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

GAL1

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-3xFLAG-HIS3MX6

Plasmid

#247614

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

3xFLAG

Expression

Yeast

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-mNeon-HISMX6

Plasmid

#247607

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

mNeonGreen

Expression

Yeast

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPIC9K_A2AR_316_V229C_R291A

Plasmid

#246917

Purpose

Express A2AR mutants in Pichia Pastoris

Depositor

Libin Ye

Publication

Wang et al Nat Commun. 2023 Mar 10;14(1):1325. doi:

Insert

Adenosine A2A receptor (ADORA2A Human)

Tags

10xHis and alpha-factor secretion signal, FLAG

Expression

Yeast

Mutation

truncated to aa 1-316, V229C, R291A

Promoter

AOX1

Available since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2679

Plasmid

#241724

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSD marker for selection on blasticidin S

Depositor

Hiten Madhani

Publication

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2681

Plasmid

#241726

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

Depositor

Hiten Madhani

Publication

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2673

Plasmid

#241720

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSD marker for selection on blasticidin S

Depositor

Hiten Madhani

Publication

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAL005

Plasmid

#233865

Purpose

MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRW404-2µ-BXB1

Plasmid

#235976

Purpose

2µ plasmid for constitutive expression of BXB1 under RNR2 promoter.

Depositor

Chang Liu

Publication

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Insert

BXB1

Expression

Yeast

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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Lifeact-3xmNG::TRP1

Plasmid

#227593

Purpose

Integration vector for expressing Lifeact-3xmNeonGreen

Depositor

Bruce Goode

Publication

Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

Insert

Lifeact-3xmNeonGreen::TRP1

Tags

3xmNeonGreen

Expression

Yeast

Mutation

Truncated ABP140 (aa 1-17 only)

Promoter

Abp140

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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