8,418 results
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PADH1-TIR1-9myc-URA3
Plasmid#207016PurposeExpresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).DepositorInsertTIR1
Tags9xMycExpressionYeastPromoterpADH1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP
Plasmid#166140PurposeEGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorInsertEGFP
ExpressionYeastPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-pT231 scFv-GFP
Plasmid#218827PurposeYeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFPDepositorInsertpT231 tau scFv
ExpressionYeastPromoterGAL1-10Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ClhN-pADH3-4V5-APEX2-URA
Plasmid#207063PurposeBackbone plasmid containing ADH3 promoter and 4V5-APEX2 tag.DepositorInsert4xV5-APEX2
ExpressionYeastPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
ExpressionYeastPromoterAOX1 promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
ExpressionYeastAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
ExpressionBacterial and YeastPromoterGAL1 GAPDHAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
Tags40aa Linker, mNeonGreenExpressionYeastPromoterCYC1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ235: pRS426-His-MBP-GtFz1_Native
Plasmid#205254PurposeS. cerevisiae expression vector for GtFz1 native guideDepositorInsertGtFz1
Tags10xHis, MBPExpressionYeastAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv2
Plasmid#188604PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM2
Plasmid#198413PurposeExpress mAID-Nb(LaM2) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM2)
ExpressionYeastPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
ExpressionYeastPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
ExpressionYeastAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10p CAS9 Gal1/10p I-SceI
Plasmid#182519PurposeGalactose-Induced Expression vector for Cas9 and I-SceI.DepositorInsertsI-SceI
Cas9
ExpressionYeastAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiFC3
Plasmid#190048PurposeBiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)DepositorInsertYemVenus (ATG-aa173-238)
ExpressionYeastPromoterMet3Available sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiFC4
Plasmid#190049PurposeBiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)DepositorInsertYemVenus (ATG-aa173-238)
ExpressionYeastPromoterMet3Available sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiFC2
Plasmid#190047PurposeBiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)DepositorInsertYemVenus (aa1-172 - TAA)
ExpressionYeastPromoterMet3Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only
