7,933 results

1. pDRF1-GW yAT1.03R122KR126K

   Plasmid

   #132782

   Purpose

   Non-binding version of the FRET biosensor for ATP measurements in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

   Insert

   yAT1.03R122KR126K ymTq2-tdTomato
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R122K, R126K

   Promoter

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. ZJOM113

   Plasmid

   #133666

   Purpose

   Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRMH122-LexA-CRY2PHR

   Plasmid

   #40613

   Purpose

   Expresses LexA-CRY2PHR for light-inducible protein expression when coupled with VP16-CIB

   Depositor

   Chandra Tucker

   Article

   Hughes et al Methods. 2012 Aug 15.

   Insert

   LexA-CRY2PHR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK_P-GAP-ACC1*

   Plasmid

   #126740

   Purpose

   yeast plasmid overexpressing ACC1 from Komagataella phaffii

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acc1*
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T3452G

   Promoter

   GAP

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDM291-tetR-tup11Δ70

   Plasmid

   #41027

   Depositor

   Michael Nick Boddy

   Article

   Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

   Insert

   tetR-tup11Δ70
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   tup11 starts at aa70

   Promoter

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS315e_pGal-dCas9-PmCDA1

   Plasmid

   #79615

   Purpose

   Expresses dCas9-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p112A1NE

   Plasmid

   #85983

   Purpose

   For construction of cDNA expression library.

   Depositor

   Wolf Frommer

   Article

   Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Adh1

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYX233

   Plasmid

   #163762

   Purpose

   Empty vector for Gal-inducible yeast expression

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Type

   Empty backbone

   Use

   Tags

   Hemagglutinin (HA) tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBD-pZ-FLAG

   Plasmid

   #183148

   Purpose

   Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Insert

   GAL4 BD
   

   Use

   Yeast two-hybrid

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-SKII-3XHA-HALO-Hygromycin

    Plasmid

    #188929

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-Hygromycin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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