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Yeast Expression

7,941 results

Showing: 5451 - 5500 of 7941 results

  1. pGBT9-GGA2-GAE

    Plasmid
    #199175
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.
    Insert
    GGA2-GAE domain (GGA2 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Contains Lys 545 Gln substitution
    Promoter
    ADH1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199175-plasmid-map-sequence-id-389860

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  6. pGBT9-ATG9A-N

    Plasmid
    #198530
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11
    Insert
    ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198530-plasmid-map-sequence-id-389380

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  9. pGBT9-Hook1

    Plasmid
    #198523
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979
    Insert
    Hook1 (HOOK1 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent substitution in codon 620 to eliminate int…
    Promoter
    ADH1
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198523-plasmid-map-sequence-id-389375

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  10. pGADT7-δ

    Plasmid
    #197259
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-3 δ (AP3D1 Human)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Val 549 Ile substitution; silent substitutions in…
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197259-plasmid-map-sequence-id-387016

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  13. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197513-plasmid-map-sequence-id-388877

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  14. pGADT7-αC

    Plasmid
    #197650
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-2 αC
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Ser677T substitution
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197650-plasmid-map-sequence-id-388876

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  16. pGADT7-AP-4 β4

    Plasmid
    #198327
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-4 β4
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Contains L480S substitution
    Promoter
    ADH1
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198327-plasmid-map-sequence-id-387831

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  18. pGADT7-AP-2 σ2

    Plasmid
    #198345
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-2 σ2
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    R42G substitution, and silent substitutions in co…
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198345-plasmid-map-sequence-id-387832

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  19. pGADT7-AP-1 σ1B

    Plasmid
    #198335
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1B
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198335-plasmid-map-sequence-id-387734

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  20. pGADT7-AP-1 σ1A

    Plasmid
    #198334
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198334-plasmid-map-sequence-id-387458

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  21. pGADT7-AP-1 σ1C

    Plasmid
    #198336
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1C
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    S148C substitution
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198336-plasmid-map-sequence-id-387637

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  24. pACT2-μ1B (137-423)

    Plasmid
    #198172
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-1 μ1B (137-423)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198172-plasmid-map-sequence-id-387535

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  26. pACT2-μ1A

    Plasmid
    #198168
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-1 μ1A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198168-plasmid-map-sequence-id-387525

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  27. pACT2-μ2

    Plasmid
    #198173
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-2 μ2
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198173-plasmid-map-sequence-id-387524

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  32. pGADT7-γ1

    Plasmid
    #197078
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-1 γ1 (Ap1g1 Mouse)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197078-plasmid-map-sequence-id-387046

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  33. pGADT7-ε

    Plasmid
    #197261
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-4 ε (AP4E1 Human)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitutions in codons 905, 1129 and 1137
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197261-plasmid-map-sequence-id-387043

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  40. pCAS-mak10 gRNA-MAK10Δ

    Plasmid
    #160384
    Purpose
    For CRISPR-mediated deletin og MAK10 in yeast
    Depositor
    John McCusker
    Insert
    MAK10
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    160384-plasmid-map-sequence-id-384173

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  41. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    John McCusker
    Insert
    mak10 gRNA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    160385-plasmid-map-sequence-id-384166

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  50. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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Showing: 5451 - 5500 of 7941 results