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ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv

Plasmid

#183733

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB6637

Plasmid

#212696

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13089

Plasmid

#212676

Purpose

TEF promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

TEF

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12706

Plasmid

#212677

Purpose

EXP promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

EXP

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12708

Plasmid

#212678

Purpose

GPD promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

GPD

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB13090

Plasmid

#212679

Purpose

Lip2 terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

Lip2

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12710

Plasmid

#212680

Purpose

Syn8 terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

Syn8

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PCfB12709

Plasmid

#212681

Purpose

SynGuo terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

SynGuo

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8860

Plasmid

#212697

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8856

Plasmid

#212701

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8866

Plasmid

#212703

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13014

Plasmid

#212685

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6630

Plasmid

#212700

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Expression

Yeast

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6638

Plasmid

#212698

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Expression

Yeast

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pTGL3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pPEX3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12718

Plasmid

#212692

Purpose

integration vector for three gene cassette with integration site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12715

Plasmid

#212689

Purpose

integration vector for three gene cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12712

Plasmid

#212683

Purpose

integration vector for single cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13017

Plasmid

#212693

Purpose

integration vector for three gene cassette with integration site of the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12711

Plasmid

#212682

Purpose

integration vector for single cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13015

Plasmid

#212686

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13016

Plasmid

#212687

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12713

Plasmid

#212684

Purpose

integration vector for single cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12716

Plasmid

#212690

Purpose

integration vector for three gene cassette with integration site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12714

Plasmid

#212688

Purpose

integration vector for three gene cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PCUP1-4V5-Pho8-Met

Plasmid

#207029

Purpose

Expression of 4V5-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xV5

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
BS-Trp-PCUP1-4myc-Pho8

Plasmid

#207028

Purpose

Expression of 4Myc-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xMyc

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PCUP1-GFP-Vma13-Ura

Plasmid

#207026

Purpose

Expression of GFP-Vma13 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vma13 ORF

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHAP8

Plasmid

#206857

Purpose

A plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHAP7

Plasmid

#206853

Purpose

A plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPt_Sap

Plasmid

#206856

Purpose

A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

mRuby3

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pXOOY

Plasmid

#196449

Purpose

A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

Depositor

Per Amstrup Pedersen

Article

Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

Type

Empty backbone

Use

Expression in xenopus laevis

Expression

Yeast

Promoter

yeast CYC-GAL and T7 promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pTPI1-Emc1-GFP-APEX2-Ura

Plasmid

#207074

Purpose

Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Emc1

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pTPI1-Cox4-GFP-APEX2-Ura

Plasmid

#207075

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
IAA17-4flag-HIS5

Plasmid

#207015

Purpose

For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(48)-NLS-VSV

Plasmid

#212746

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(63)-VSV

Plasmid

#212752

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-TET-NUbo-E3(63)-VSV

Plasmid

#212753

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(63)-NLS-VSV

Plasmid

#212756

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-TET-NUbo-E3(48)-VSV

Plasmid

#212744

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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