8,436 results

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EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Publication

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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cyterm-ymtq2 pDRF1-GW

Plasmid

#168083

Purpose

Expresses Cyterm-ymTq2 to check for monomericity.

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymTq2

Expression

Yeast

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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LifeAct-mScarletI pDRF1-GW

Plasmid

#168085

Purpose

Expresses Lifeact-mScarletI to visualize actin in yeast

Depositor

Bas Teusink

Insert

LifeAct-mScarletI

Expression

Yeast

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNA0304

Plasmid

#165612

Purpose

Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

Depositor

Marcus Noyes

Publication

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)

Use

CRISPR

Expression

Yeast

Mutation

NotI site replaced the CAN1 spacer in parent vect…

Promoter

SNR52

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

Plasmid

#169353

Purpose

Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

Depositor

Tony Carr

Insert

OsTIR1(F74A)

Expression

Yeast

Mutation

F74A

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGY416

Plasmid

#166660

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

Depositor

Gaël Yvert

Publication

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

GAL1

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Publication

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pVG49

Plasmid

#164707

Purpose

yEVenus under the control of GAL1 promoter

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

GAL1pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-URA

Plasmid

#160015

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Expression

Yeast

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase

Plasmid

#162556

Purpose

NheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.

Depositor

Adnane Sellam

Publication

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Expression

Yeast

Promoter

Actine

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pPAP001

Plasmid

#153488

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-MNase-CaARG4

Plasmid

#162463

Purpose

DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4

Depositor

Adnane Sellam

Publication

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pYTSG

Plasmid

#159419

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Yeast

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Publication

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pI6-MTU-DO-URA

Plasmid

#160030

Purpose

Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pYM29-BirA*

Plasmid

#160291

Purpose

Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1

Depositor

Martin Ott

Publication

Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

Insert

BirA*

Expression

Yeast

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-HIS

Plasmid

#160142

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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