8,043 results
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Plasmid#234121PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationS1584YPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 V1862L
Plasmid#234123PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationV1862LPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IHA
Plasmid#232993PurposeGalactose iduced expression of Gcn4 F124IHA in yeastDepositorInsertGcn4 F124I
UseTags3xHA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_FL Gcn4
Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-16
Plasmid#234138PurposeFirst 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del103-173
Plasmid#234134PurposeAmino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationC-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6-CFLAG
Plasmid#234133PurposeRestriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del134-173
Plasmid#234135PurposeAmino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationC-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6
Plasmid#234140PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-NFLAG-Fyv6
Plasmid#234139PurposeRestriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 G1868R
Plasmid#234124PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationG1868RPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Fyv6
Plasmid#234141PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-23
Plasmid#234137PurposeFirst 23 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-M175I
Plasmid#234118PurposeSuppressor screen mutant in Cef2DepositorInsertCef1
UseTagsExpressionYeastMutationM175IPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-Q193P
Plasmid#234117PurposeSuppressor screen mutant in Cef1DepositorInsertCef1
UseTagsExpressionYeastMutationQ193PPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-A37V
Plasmid#234119PurposeSuppressor screen mutant in Cef3DepositorInsertCef1
UseTagsExpressionYeastMutationA37VPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Syf1
Plasmid#234113PurposeSyf1 region PCR, restriction digest and ligation into pRS416 backboneDepositorInsertSyf1
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 634 Truncation
Plasmid#234116PurposeSyf1 PCR truncation starting at 634 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7_Sr62NLRa
Plasmid#233528PurposeExpresses Sr62NLRa in yeastDepositorInsertSr62NLRa
UseTagsGAL4 AD, HA tagExpressionYeastMutationPromoterADH1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1
Plasmid#234114PurposeSyf1 region PCR, restriction digest and ligation into pRS414 backboneDepositorInsertSyf1
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 778 Truncation
Plasmid#234115PurposeSyf1 PCR truncation starting at 778 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WTSLFD
Plasmid#232980PurposeGalactose iduced expression of Gcn4 WTSLFD+ in yeastDepositorInsertGcn4 WTSLFD+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+
Plasmid#232982PurposeGalactose iduced expression of Gcn4 Aro2+ in yeastDepositorInsertGcn4 Aro2+
UseTagsTEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+
Plasmid#232983PurposeGalactose iduced expression of Gcn4 Aro3+ in yeastDepositorInsertGcn4 Aro3+
UseTagsTEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AHA
Plasmid#232992PurposeGalactose iduced expression of Gcn4 W120AHA in yeastDepositorInsertGcn4 W120A
UseTags3xHA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRHA
Plasmid#232994PurposeGalactose iduced expression of Gcn4 SATtoG KtoRHAin yeastDepositorInsertGcn4 SATtoG KtoR
UseTags3xHA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+HA
Plasmid#232990PurposeGalactose iduced expression of Gcn4 Aro3+HA in yeastDepositorInsertGcn4 Aro3+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WT 44merHA
Plasmid#232988PurposeGalactose iduced expression of Gcn4 WT 44merHA in yeastDepositorInsertGcn4 WT 44mer
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+HA
Plasmid#232989PurposeGalactose iduced expression of Gcn4 Aro2+HA in yeastDepositorInsertGcn4 Aro2+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IGFP
Plasmid#233005PurposeGalactose iduced expression of Gcn4 F124IGFP in yeastDepositorInsertGcn4 F124I
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+HA
Plasmid#232991PurposeGalactose iduced expression of Gcn4 WW+HA in yeastDepositorInsertGcn4 WW+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 L123A
Plasmid#232969PurposeGalactose iduced expression of Gcn4 L123A in yeastDepositorInsertGcn4 L123A
UseTagsTEV cleavage siteExpressionYeastMutationL123APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoR
Plasmid#232961PurposeGalactose iduced expression of Gcn4 ATtoG KtoR in yeastDepositorInsertGcn4 SATtoG KtoR
UseTagsTEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap2
Plasmid#232965PurposeGalactose iduced expression of Gcn4 k0.19 in yeastDepositorInsertGcn4 k0.19
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+GFP
Plasmid#233003PurposeGalactose iduced expression of Gcn4 WW+GFP in yeastDepositorInsertGcn4 WW+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AGFP
Plasmid#233004PurposeGalactose iduced expression of Gcn4 120AGFP in yeastDepositorInsertGcn4 W120A
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+GFP
Plasmid#233001PurposeGalactose iduced expression of Gcn4 Aro2+GFP in yeastDepositorInsertGcn4 Aro2+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol
Plasmid#232976PurposeGalactose iduced expression of Gcn4 solvvol in yeastDepositorInsertGcn4 solvvol
UseTagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 S/ArotoL
Plasmid#232979PurposeGalactose iduced expression of Gcn4 S/ArotoL in yeastDepositorInsertGcn4 S/ArotoL
UseTagsTEV cleavage siteExpressionYeastMutationS101L, S104L, F108L, Y110L, S117L, S136L, S144LPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only