8,173 results
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Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc
Plasmid#199760PurposeElongation reporter construct to quantify elongation duration of 6_CGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[CGA]-nLuc
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc
Plasmid#199761PurposeElongation reporter construct to quantify elongation duration of 6_AGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[AGA]-cLuc
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 6FLAG
Plasmid#182509PurposeC-SWAT compatible vector for C-terminal tagging with 6 FLAGDepositorInsert6 FLAG
ExpressionYeastAvailable SinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG (no NLS)
Plasmid#193322PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..DepositorInsertIAA17
Tags6FLAGExpressionYeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M2C5
Plasmid#188606PurposeMinimal Conjugative Plasmid 2DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M1C1
Plasmid#188609PurposeMinimal Conjugative Plasmid 1DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M4C16
Plasmid#188610PurposeMinimal Conjugative Plasmid 4DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M4C19
Plasmid#188611PurposeMinimal Conjugative Plasmid 4DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
ExpressionYeastPromoterCUP1 promoterAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ4
Plasmid#198176PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 μ4
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymTurquoise2
Plasmid#193958PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeastDepositorInsertymTurquoise2
ExpressionYeastPromoterTEF1Available SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA2-GAE
Plasmid#199175PurposeExpression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA2-GAE domain (GGA2 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationContains Lys 545 Gln substitutionPromoterADH1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-γ1 ear
Plasmid#199177PurposeExpression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 γ1 ear domain (Ap1g1 Mouse)
TagsGAL4-DNA binding domain fragmentExpressionYeastPromoterADH1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA3-GAE
Plasmid#199176PurposeExpression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA3-GAE domain (GGA3 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastPromoterADH1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-γ2 ear
Plasmid#199178PurposeExpression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 γ2 ear domain (AP1G2 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastPromoterADH1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA1-GAE
Plasmid#199174PurposeExpression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA1-GAE domain (GGA1 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastPromoterADH1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-ATG9A-N
Plasmid#198530PurposeExpression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastPromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-FTS
Plasmid#198526PurposeExpression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)DepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-FHIP-L
Plasmid#198528PurposeExpression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)DepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook1
Plasmid#198523PurposeExpression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook1 (HOOK1 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 620 to eliminate int…PromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-δ
Plasmid#197259PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-3 δ (AP3D1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationVal 549 Ile substitution; silent substitutions in…PromoterADH1Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-Rabex-5
Plasmid#197043PurposeExpression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertRabex-5 (RABGEF1 Bovine)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
TagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS_phleo-Tps1/Gsy2
Plasmid#196612PurposeEncoding guide RNAs for the knock out of TPS1 and GSY2 genesDepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-4 β4
Plasmid#198327PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 β4
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationContains L480S substitutionPromoterADH1Available SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-AP-4 σ4
Plasmid#198328PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 σ4
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-2 σ2
Plasmid#198345PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-2 σ2
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationR42G substitution, and silent substitutions in co…PromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1B
Plasmid#198335PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1B
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1A
Plasmid#198334PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1C
Plasmid#198336PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1C
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationS148C substitutionPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3A
Plasmid#198347PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3B
Plasmid#198348PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3B
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1B (137-423)
Plasmid#198172PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1B (137-423)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOAD eIF4G1 (1-341)
Plasmid#196833PurposeExpesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.DepositorInserteIF4G1 (TIF4631 Budding Yeast)
TagsGal4ExpressionYeastMutationamino acids 1-341PromoterADHAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1A
Plasmid#198168PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ2
Plasmid#198173PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-2 μ2
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-AAGAB
Plasmid#198332PurposeExpression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAAGAB (AAGAB Human)
TagsGAL4-DNA binding domain (BD) fragmentExpressionYeastPromoterADH1Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 WT (123-204)
Plasmid#196834PurposeExpresses Pab1 RRM2 fragment fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
TagsGal4ExpressionYeastMutation123-204 amino acidsPromoterADHAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 E181R (123-204)
Plasmid#196835PurposeExpresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
TagsGal4ExpressionYeastMutation123-204 amino acids & E181RPromoterADHAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS13-Tps2/Gsy1
Plasmid#196613PurposeEncoding guide RNAs for the knock out of TPS2 and GSY1 genesDepositorAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-γ1
Plasmid#197078PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-1 γ1 (Ap1g1 Mouse)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ε
Plasmid#197261PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-4 ε (AP4E1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitutions in codons 905, 1129 and 1137PromoterADH1Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-tepsin
Plasmid#196647PurposeExpression of GAL4 DNA-binding domain (BD)-tepsin fusion proteinDepositorInserttepsin (TEPSIN Human)
UseYeast two-hybrid vectorTagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 199 to eliminate int…Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUGP1.1
Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-PAH1-3HA
Plasmid#184761PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-CDS1-3HA
Plasmid#184760PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-DGK1-3HA
Plasmid#184759PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS-mak10 gRNA-MAK10Δ
Plasmid#160384PurposeFor CRISPR-mediated deletin og MAK10 in yeastDepositorInsertMAK10
ExpressionYeastPromoterPGK1Available SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only