7,935 results

1. pAG426-GAL-Alpha-synuclein-A53T

   Plasmid

   #181722

   Purpose

   Galactose inducible expression of Alpha-synuclein-A53T

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A53T

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-SKII-URA-GAL1-fCLIP-3xHA

   Plasmid

   #188936

   Purpose

   Integration cassette to create N-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   URA-GAL1-fCLIP-3xHA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-SKII-3XHA-fCLIP-NAT

   Plasmid

   #188921

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fCLIP-NAT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS416-PHO5-GFP-Osh1

   Plasmid

   #58835

   Purpose

   Expresses GFP-Tagged Osh1 in yeast

   Depositor

   Tim Levine

   Article

   Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

   Insert

   Osh1 (SWH1, OSH1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pMTU-DO-URA-C4

   Plasmid

   #160339

   Purpose

   High-copy expression vector for K. marxianus, uracil selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScDD2_ERdd

   Plasmid

   #109047

   Purpose

   destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

   Depositor

   Thomas Wandless

   Insert

   ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
   

   Use

   Tags

   yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

   Expression

   Yeast

   Mutation

   3 mutations : E380G, R434G, N532S

   Promoter

   SOD1

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWS3867

   Plasmid

   #185744

   Purpose

   Sub-array backbone 3/4

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS712

   Plasmid

   #213041

   Purpose

   CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

   Depositor

   Rebecca Shapiro

   Article

   Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. C-SWAT AID*-GFP osTIR1

   Plasmid

   #182514

   Purpose

   C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   AID*
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pI3-MTU-DO-G418

    Plasmid

    #160023

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge