8,043 results

1. pAG415Gal_Gcn4 K5

   Plasmid

   #232966

   Purpose

   Galactose iduced expression of Gcn4 K+ in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 K+
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   D103K, D115K, D127K, D133K, E143K

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG415Gal_Gcn4 ILVtoED solvvol

   Plasmid

   #232958

   Purpose

   Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED solvvol
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG415Gal_Gcn4 Swap3

   Plasmid

   #232960

   Purpose

   Galactose iduced expression of Gcn4 k0.47 in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 k0.47
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG415Gal_Gcn4 R2

   Plasmid

   #232970

   Purpose

   Galactose iduced expression of Gcn4 R+ in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 R+
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   K118R, K140R

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG415Gal_Gcn4 E+

   Plasmid

   #232963

   Purpose

   Galactose iduced expression of Gcn4 E+in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 E+
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   D105E, D118E, D139E

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG415Gal_Gcn4 W120A

   Plasmid

   #232962

   Purpose

   Galactose iduced expression of Gcn4 W120A in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 W120A
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   W120A

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG415Gal_Gcn4 P+

   Plasmid

   #232971

   Purpose

   Galactose iduced expression of Gcn4 Proline in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 Proline
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S122P, T131F, A141P, E143D

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG415Gal_Gcn4 R3

   Plasmid

   #232973

   Purpose

   Galactose iduced expression of Gcn4 R++ in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 R++
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   D103R, D133R, K143R

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG415Gal_Gcn4 Swap3GFP

   Plasmid

   #233010

   Purpose

   Galactose iduced expression of Gcn4 k0.47GFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 k0.47
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG415Gal_Gcn4 ILVtoED WHA

    Plasmid

    #232995

    Purpose

    Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG415Gal_Gcn4 9acidAHA

    Plasmid

    #232997

    Purpose

    Galactose iduced expression of Gcn4 9acidAHA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG415Gal_DBD

    Plasmid

    #232985

    Purpose

    Galactose iduced expression of Gcn4 DBD in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 DBD
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG415Gal_Gcn4 F124I

    Plasmid

    #232964

    Purpose

    Galactose iduced expression of Gcn4 F124I in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 F124I
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    F124I

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG415Gal_Gcn4 9acidA

    Plasmid

    #232967

    Purpose

    Galactose iduced expression of Gcn4 9acidA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG415Gal_Gcn4 E2K2

    Plasmid

    #232968

    Purpose

    Galactose iduced expression of Gcn4 EK+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 EK+
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S104K, S117E, S136E, S144K

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG415Gal_Gcn4 9acidAGFP

    Plasmid

    #233009

    Purpose

    Galactose iduced expression of Gcn4 9acidAGFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ppAG415Gal_Gcn4 L113A+

    Plasmid

    #232972

    Purpose

    Galactose iduced expression of Gcn4 L113A in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 L113A
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    L113A, E114Y, N126H, D127E, S136A

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG415Gal_Gcn4 Aro3+GFP

    Plasmid

    #233002

    Purpose

    Galactose iduced expression of Gcn4 Aro3+GFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG415Gal_Gcn4 ILVtoED

    Plasmid

    #232957

    Purpose

    Galactose iduced expression of Gcn4 ILVtoED in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    L123D, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG415Gal_Gcn4 Swap3HA

    Plasmid

    #232998

    Purpose

    Galactose iduced expression of Gcn4 k0.47HA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 k0.47
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAG415Gal_Gcn4 StoL

    Plasmid

    #232981

    Purpose

    Galactose iduced expression of Gcn4 StoL in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 StoL
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S104L, S117L, S136L, S144L

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAG415Gal_Gcn4 SATtoG KtoRGFP

    Plasmid

    #233006

    Purpose

    Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAG415Gal_Gcn4 ILVtoED WGFP

    Plasmid

    #233007

    Purpose

    Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG415Gal_Gcn4 R3W

    Plasmid

    #232975

    Purpose

    Galactose iduced expression of Gcn4 R++ W+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 R++ W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103R, N126W, D133R, K143R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG415Gal_Gcn4 WT 44mer

    Plasmid

    #232956

    Purpose

    Galactose iduced expression of Gcn4 WT 44mer in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WT 44mer
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG415Gal_Gcn4 E3HA

    Plasmid

    #232996

    Purpose

    Galactose iduced expression of Gcn4 E+HA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 E+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D105E, D118E, D139E

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAG415Gal_Gcn4 WW+

    Plasmid

    #232984

    Purpose

    Galactose iduced expression of Gcn4 WW+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WW+
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    E109N, S117D, T121W, N126W, T132W

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAG415Gal_Gcn4 F108A+

    Plasmid

    #232974

    Purpose

    Galactose iduced expression of Gcn4 F108A in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 F108A
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    F108A, T121L, A141P

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG415Gal_Gcn4 solvvol W

    Plasmid

    #232977

    Purpose

    Galactose iduced expression of Gcn4 solvvol W+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG415Gal_DBDGFP

    Plasmid

    #232999

    Purpose

    Galactose iduced expression of Gcn4 DBDGFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    DBD
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAG415Gal_Gcn4 ILVtoAS

    Plasmid

    #232959

    Purpose

    Galactose iduced expression of Gcn4 ILVtoAS in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoAS
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    L123S, I128S, V130A, V135A, L137S, I142S

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG415Gal_Gcn4 WT 44merGFP

    Plasmid

    #233000

    Purpose

    Galactose iduced expression of Gcn4 WT 44merGFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WT 44mer
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAG415Gal_Gcn4 WT 30mer

    Plasmid

    #232986

    Purpose

    Galactose iduced expression of Gcn4 WT 30mer in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WT 30mer
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS424 Prp8 WT TRP1

    Plasmid

    #234120

    Purpose

    The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pML107-ARB1

    Plasmid

    #232888

    Purpose

    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ARB1 gRNA (ARB1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-FLAG-APEX2-NES-HIS3

    Plasmid

    #229230

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRS413-CUP1pr-NFAPoptim

    Plasmid

    #221110

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRS413-CUP1pr-CFAPoptim

    Plasmid

    #221111

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid

    #221114

    Purpose

    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    MFA-IgKappa-FAPoptim-STE3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST

    Plasmid

    #221118

    Purpose

    FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    MFA-IgKappa-FAPoptim-STE3-deltaPEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    STE3 mutant that lacks the PEST domain in the C-t…

    Promoter

    STE3

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge