8,043 results

1. Leu2 2u

   Plasmid

   #220622

   Purpose

   empty backbone for shuttle vector construction

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Insert

   empty vector (LEU2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RT-1

   Plasmid

   #220434

   Purpose

   Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pReporter_ZF2_ZF1_mNeonGreen

   Plasmid

   #213834

   Purpose

   Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pReporter_ZF1_mNeonGreen_ZF2

   Plasmid

   #213835

   Purpose

   Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pReporter_ZF1_ZF2_mNeonGreen

   Plasmid

   #213836

   Purpose

   Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pReporter_ZF2_mNeonGreen_ZF1

   Plasmid

   #213837

   Purpose

   Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pReporter_mNeonGreen

   Plasmid

   #213838

   Purpose

   Facilitates genomic integration of the control reporter without zinc finger binding sites.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPtet-1_mNeonGreen_Ptet-2_mCherry

   Plasmid

   #213839

   Purpose

   Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen, mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pReporter_ZF1_mNeonGreen

   Plasmid

   #213840

   Purpose

   Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pReporter_ZF2_mNeonGreen

    Plasmid

    #213841

    Purpose

    Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPtet-2-VP16

    Plasmid

    #213842

    Purpose

    Used for expressing VP16 fused to ZF-2.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPtet-1-VP16

    Plasmid

    #213843

    Purpose

    Used for expressing VP16 fused to ZF-1.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pStorage_Ptet-1_ZF1

    Plasmid

    #213844

    Purpose

    CombiGEM storage plasmid for ZF-1 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pStorage_Ptet-2_ZF2

    Plasmid

    #213845

    Purpose

    CombiGEM storage plasmid for ZF-2 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pReporter_TetR

    Plasmid

    #213847

    Purpose

    Facilitates genomic integration of tetR expression cassette.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    TetR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfb12622

    Plasmid

    #219929

    Purpose

    PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter ACT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfb13237

    Plasmid

    #219910

    Purpose

    The base plasmid of TUNEYALI for TF47

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfb12562

    Plasmid

    #219923

    Purpose

    PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter GPD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ClhN-p500-Erg6-V5-APEX2-URA

    Plasmid

    #207070

    Purpose

    Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Erg6
    

    Use

    Tags

    V5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pERG6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA

    Plasmid

    #207064

    Purpose

    Expressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Pex3
    

    Use

    Tags

    4xV5-APEX2 and GFP

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BS-ZJLEU-PTEF1-GFP

    Plasmid

    #207018

    Purpose

    Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PTPI1-Dsred-URA3

    Plasmid

    #207017

    Purpose

    Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    DsRed-Express 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTPI1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCfb13186

    Plasmid

    #219924

    Purpose

    PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter TEF
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfb13204

    Plasmid

    #219879

    Purpose

    The base plasmid of TUNEYALI for TF14

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfb13200

    Plasmid

    #219875

    Purpose

    The base plasmid of TUNEYALI forTF10

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCfb13242

    Plasmid

    #219914

    Purpose

    The base plasmid of TUNEYALI for TF52

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCfb13245

    Plasmid

    #219916

    Purpose

    The base plasmid of TUNEYALI for TF55

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF55 (YALI1_A16891g) and homologous arm matching TF55
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCfb13193

    Plasmid

    #219868

    Purpose

    The base plasmid of TUNEYALI for TF03

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCfb12561

    Plasmid

    #219922

    Purpose

    PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter PGM
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCfb12565

    Plasmid

    #219925

    Purpose

    PrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter FBA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCfb13223

    Plasmid

    #219898

    Purpose

    The base plasmid of TUNEYALI for TF33

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF33 (YALI1_F14650g) and homologous arm matching TF33
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCfb13197

    Plasmid

    #219872

    Purpose

    The base plasmid of TUNEYALI forTF07

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF07 ( YALI1_D30097g) and homologous arm matching TF07
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCfb13226

    Plasmid

    #219901

    Purpose

    The base plasmid of TUNEYALI for TF36

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF36 (YALI1_A22330g) and homologous arm matching TF36
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCfb13194

    Plasmid

    #219869

    Purpose

    The base plasmid of TUNEYALI for TF04

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF04 (YALI1_C02831g) and homologous arm matching TF04
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCfb13209

    Plasmid

    #219884

    Purpose

    The base plasmid of TUNEYALI for TF19

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCfb13208

    Plasmid

    #219883

    Purpose

    The base plasmid of TUNEYALI for TF18

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF18 (YALI1_D15792g) and homologous arm matching TF18
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCfb13207

    Plasmid

    #219882

    Purpose

    The base plasmid of TUNEYALI for TF17

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF17 (YALI1_B19962g) and homologous arm matching TF17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCfb13244

    Plasmid

    #219915

    Purpose

    The base plasmid of TUNEYALI for TF54

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF54 (YALI1_F26367g) and homologous arm matching TF54
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfb13233

    Plasmid

    #219906

    Purpose

    The base plasmid of TUNEYALI for TF43

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF43 (YALI1_C14774g) and homologous arm matching TF41
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfb13206

    Plasmid

    #219881

    Purpose

    The base plasmid of TUNEYALI for TF16

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF16 (YALI1_B11759g) and homologous arm matching TF16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge