8,060 results

1. pFA6a-link-yoPS-CFP2-SpHis5

   Plasmid

   #44853

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPS-CFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETcon(I-LtrI)

   Plasmid

   #41523

   Depositor

   Andrew Scharenberg

   Insert

   I-LtrI
   

   Use

   Tags

   3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXP732

   Plasmid

   #46058

   Depositor

   Nancy DaSilva

   Article

   Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHUM

   Plasmid

   #64170

   Purpose

   harbours marker genes HIS3, URA3 and MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-link-yoEmerald-Kan

   Plasmid

   #44946

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEmerald
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-link-yoPS-CFP2-Kan

   Plasmid

   #44948

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPS-CFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIplac211-CHC1-msGFPx3

   Plasmid

   #105256

   Purpose

   Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Chc1 portion (CHC1 Budding Yeast)
   

   Use

   Tags

   msGFPx3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYS104

   Plasmid

   #1190

   Depositor

   Susan Lindquist

   Article

   Patino et al Science 1996 Aug 2;273(5275):622-6.

   Insert

   Hsp104 (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wild-type Hsp104 sequence with 588bp of 5' p…

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pJK372

   Plasmid

   #73597

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-15 fatty acid desaturase
   

   Use

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LHP462 - Ub-HA

    Plasmid

    #32169

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pMEG_BB3_YL001

    Plasmid

    #117825

    Purpose

    Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpTEF_YlGUT1_YlCyc1TT
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GTH-y

    Plasmid

    #81105

    Purpose

    Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCitrine
    

    Use

    Cre/Lox

    Tags

    mCitrine

    Expression

    Yeast

    Mutation

    Mutated mCitrine for monomeric isoform (A206K L22…

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB8383

    Plasmid

    #126917

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    tHMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FC857

    Plasmid

    #27327

    Depositor

    Fred Cross

    Article

    Cross et al Yeast. 2011 Feb . 28(2):167-79.

    Insert

    CDH1 (CDH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C-terminal truncation of CDH1 at its BamHI site

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YCPlac33-sGFP-VRG4

    Plasmid

    #25447

    Depositor

    Benjamin Glick

    Article

    Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

    Insert

    Synthetic Green Fluorescent Protein - VRG4
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046457

    Plasmid

    #145424

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. YEplac181-TYS1

    Plasmid

    #8629

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics 2004 Mar;166(3):1215-27.

    Insert

    TYS1 (TYS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046390

    Plasmid

    #148996

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCfB3050(gRNA XII-5)

    Plasmid

    #73292

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDZ514 pURA MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72230

    Purpose

    Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge