8,418 results
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Plasmid#103844PurposeYeast Expression PlasmidDepositorInsertpAdh1-Msn2-mCherry
TagsmCherryExpressionYeastAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-URA3
Plasmid#122915PurposeTRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyterm-ymScarletI pDRF1-GW
Plasmid#168082PurposeExpresses Cyterm-ymScarletI to check for monomericity.DepositorInsertcyterm-ymScarletI
ExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAW8-aid-5Myc-Turg1:kanMX6
Plasmid#169356PurposeC-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombeDepositorInsertauxin-inducible degron (aid)
Tags5XMycExpressionYeastAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pITI1
Plasmid#166094PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-RFP
Plasmid#166841PurposeExpresses Vac8-RFP in yeastsDepositorAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRR2
Plasmid#166097PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-hENT1
Plasmid#166028PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyExpressionYeastPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP403red-hENT1
Plasmid#166027PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyExpressionYeastPromoterpTDH3Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-pluxO5-ptetO7-crtYBI
Plasmid#165975PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-rtetTAK8N, L131L
Plasmid#165968PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG45
Plasmid#164703PurposeyEVenus under the control of CUP1 promoterDepositorInsertCUP1pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG48
Plasmid#164706PurposertTA under the control of PGK1 promoterDepositorInsertPGK1pr - rtTA - CYC1t
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-URA
Plasmid#160015PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only