8,086 results

1. pGBW-m4046457

   Plasmid

   #145424

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046390

   Plasmid

   #148996

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pXP822

   Plasmid

   #46064

   Depositor

   Nancy DaSilva

   Article

   Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. RPL25NLS-GFP (101)

   Plasmid

   #24038

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Only bp 1-135 (aa 1-45) of RPL25

   Promoter

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGBW-m4046332

   Plasmid

   #145413

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. EZ-L767

   Plasmid

   #131773

   Purpose

   PADH1_sFUS_Fusion Red_PixD_TACT1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PADH1_sFUS_Fusion Red_PixD_TACT1
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PADH1

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p405GALL

   Plasmid

   #15964

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   p405GALL (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALL promoter is between SacI and XbaI restrictio…

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUDP003

   Plasmid

   #101164

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FC857

   Plasmid

   #27327

   Depositor

   Fred Cross

   Article

   Cross et al Yeast. 2011 Feb . 28(2):167-79.

   Insert

   CDH1 (CDH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C-terminal truncation of CDH1 at its BamHI site

   Promoter

   Available Since

   Jan. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pR208IIM1

    Plasmid

    #45352

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

    Insert

    UBR1 mutant
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    P406S, DE317NK

    Promoter

    ADH

    Available Since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

11. YIplac211-iGFP-YPT31

    Plasmid

    #105263

    Purpose

    Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Ypt31 (YPT31 )
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid

    #169355

    Purpose

    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    3XHA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW8-aid-Turg1:kanMX6

    Plasmid

    #169354

    Purpose

    C-terminal aid degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YEplac181-TYS1

    Plasmid

    #8629

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics 2004 Mar;166(3):1215-27.

    Insert

    TYS1 (TYS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available Since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFS177

    Plasmid

    #12380

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    human equilibrating nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N81S and M84V

    Promoter

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLK89 - Edit-directing plasmid cloning insert

    Plasmid

    #98846

    Purpose

    Contains the insert used for step 2 of edit-directed plasmid cloning

    Depositor

    Leonid Kruglyak

    Insert

    KanR
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Changed sequence to remove a MluI cut site

    Promoter

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCri-15a

    Plasmid

    #61323

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRPR1_c3gRNA_RPR1t

    Plasmid

    #64381

    Purpose

    encodes c3 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c3 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pRB2960

    Plasmid

    #8677

    Depositor

    David Botstein

    Article

    Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

    Insert

    GFP::TUB1 under GAL1 promoter (TUB1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pRS415-SOH1

    Plasmid

    #8622

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics 2004 Mar;166(3):1215-27.

    Insert

    SOH1 (SOH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available Since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge