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pICE TRP1-NatMx

Plasmid

#122903

Purpose

TRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPAP002

Plasmid

#153489

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA-MNase-CaURA3

Plasmid

#162461

Purpose

DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3

Depositor

Adnane Sellam

Publication

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBP-4_1.sensor

Plasmid

#162844

Purpose

Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Publication

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1_RNA-device

Expression

Yeast

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFBP-4_1m.mutant

Plasmid

#162869

Purpose

Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Publication

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1m_RNA-device

Expression

Yeast

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GYB

Plasmid

#160927

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-Aga1p-FAPB2.3.6

Plasmid

#158147

Purpose

Yeast display reporter for non-display yeast strains (wild-type construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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VanR-wt

Plasmid

#153339

Purpose

Wild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoter

Depositor

Michael Jensen

Publication

et al Biotechnology Notes, Volume 1, 2020, Pag

Insert

VanR

Expression

Yeast

Promoter

TEF1

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-MNase-CaHIS1

Plasmid

#162462

Purpose

DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1

Depositor

Adnane Sellam

Publication

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTIN-ura4

Plasmid

#161808

Purpose

A ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe

Depositor

Stewart Shuman

Type

Empty backbone

Expression

Yeast

Promoter

Pho1

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-LEU

Plasmid

#160013

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBAC690-TEF

Plasmid

#160432

Purpose

Expresses eGFP under control of the yeast TEF2 promoter

Depositor

Gary Stormo

Publication

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

S. cerevisiae TEF2 promoter

Expression

Yeast

Promoter

S. cerevisiae TEF2

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-HPH

Plasmid

#160207

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-LEU

Plasmid

#160176

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-LEU

Plasmid

#160029

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-HIS

Plasmid

#160021

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-HIS

Plasmid

#160016

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMJB1-RPS22B-Cl

Plasmid

#160431

Purpose

Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

Depositor

Gary Stormo

Publication

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

RPS22B (RPS22B Budding Yeast)

Tags

mCherry

Expression

Yeast

Mutation

Removed introns, shuffled codons to change nucleo…

Promoter

GAL1 and S. cerevisiae GAL1

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMJB1

Plasmid

#160429

Purpose

Yeast vector for expressing mCherry tagged genes under the GAL1 promoter

Depositor

Gary Stormo

Publication

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Promoter

GAL1

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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