8,060 results

1. pVW284

   Plasmid

   #139494

   Purpose

   Expression vector for the PP7-GFP protein in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pADH1 PP7∆FG-GFPenvy tCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ∆FG

   Promoter

   pADH1

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVW300

   Plasmid

   #139495

   Purpose

   Expression vector for the PP7-GFP protein in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pTEF PP7∆FG-GFPenvy tCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ∆FG

   Promoter

   pTEF

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AtCOPT1 upGFP

   Plasmid

   #117899

   Purpose

   protein expression

   Depositor

   Geoffrey Chang

   Insert

   AtCOPT1 upGFP (COPT1 Mustard Weed)
   

   Use

   Tags

   eGFP

   Expression

   Yeast

   Mutation

   Promoter

   AOX

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCri-15a

   Plasmid

   #61323

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB4132

   Plasmid

   #106127

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ZJOM108

   Plasmid

   #133665

   Purpose

   Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV0479

   Plasmid

   #133499

   Purpose

   Expression of sfGFP excluded from the nucleus with two NES signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS415-SOH1

   Plasmid

   #8622

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics 2004 Mar;166(3):1215-27.

   Insert

   SOH1 (SOH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Promoter

   Available since

   Nov. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Osa-pCAMBIA-1301-STTM159

   Plasmid

   #84195

   Purpose

   Disturb the expression of miR159 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR159
   

   Use

   Tags

   Expression

   Bacterial, Plant, and Yeast…

   Mutation

   Promoter

   2x35S

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pRPR1_c3gRNA_RPR1t

    Plasmid

    #64381

    Purpose

    encodes c3 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c3 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRS424-TUB1-Cterminal6xHis

    Plasmid

    #60387

    Purpose

    Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    6xHIS

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

12. JME4393

    Plasmid

    #129657

    Purpose

    LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFS177

    Plasmid

    #12380

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    human equilibrating nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N81S and M84V

    Promoter

    Available since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV0543

    Plasmid

    #133493

    Purpose

    Expression of nucleo-cytoplasmic mCherry in P-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSK247

    Plasmid

    #131148

    Purpose

    dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSUP35, min pCYC1

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRB2960

    Plasmid

    #8677

    Depositor

    David Botstein

    Article

    Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

    Insert

    GFP::TUB1 under GAL1 promoter (TUB1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pGWKS12

    Plasmid

    #139419

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Superfolder GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p42Nat 3-F GW

    Plasmid

    #58557

    Purpose

    Multicopy with clonNat resistance marker for triple Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pINER2R

    Plasmid

    #185882

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pITI1

    Plasmid

    #166094

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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