8,043 results

1. pEM.F01R

   Plasmid

   #198660

   Purpose

   Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with HI protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEM.F01L

   Plasmid

   #198659

   Purpose

   Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with CD protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEM.A02R

   Plasmid

   #198658

   Purpose

   Easy-MISE toolkit pEM-plasmid containing a spacer sequence with FL protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   Adaptor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEM.A02L

   Plasmid

   #198657

   Purpose

   Easy-MISE toolkit pEM-plasmid containing a spacer sequence with BF protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   Adaptor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEM.A01R

   Plasmid

   #198656

   Purpose

   Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   Adaptor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEM.A01L

   Plasmid

   #198655

   Purpose

   Easy-MISE toolkit pEM-plasmid containing a spacer sequence with CD protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   Adaptor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEM.T02R

   Plasmid

   #198654

   Purpose

   Easy-MISE toolkit pEM-plasmid containing CYC1 terminator with IL protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   tCYC1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEM.T01L

   Plasmid

   #198653

   Purpose

   Easy-MISE toolkit pEM-plasmid containing ADH1 terminator with BC protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   tADH1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEM.P06R

   Plasmid

   #198652

   Purpose

   Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with FG protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   pPDA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEM.P06L

    Plasmid

    #198651

    Purpose

    Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pPDA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEM.P05R

    Plasmid

    #198650

    Purpose

    Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pCYC1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEM.P05L

    Plasmid

    #198649

    Purpose

    Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pCYC1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEM.P04R

    Plasmid

    #198648

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTPI1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEM.P04L

    Plasmid

    #198647

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTPI1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEM.P03R

    Plasmid

    #198646

    Purpose

    Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEM.P03L

    Plasmid

    #198645

    Purpose

    Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEM.P02R

    Plasmid

    #198644

    Purpose

    Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pENO2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEM.P02L

    Plasmid

    #198643

    Purpose

    Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pENO2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEM.P01R

    Plasmid

    #198642

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTDH3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEM.P01L

    Plasmid

    #198641

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTDH3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Aga2p-LOV-Turbo1-myc_pCTCON2

    Plasmid

    #199673

    Purpose

    expresses pre-evolved version of LOV-Turbo on the yeast surface

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo1
    

    Use

    Tags

    Aga2p and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGBT9-FHIP

    Plasmid

    #198527

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FHIP (FHIP1B Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent mutations in codons 353 and 651 as well as…

    Promoter

    ADH1

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOBD2

    Plasmid

    #196826

    Purpose

    Yeast two-hybrid ; Gal4 DNA binding domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 DNA binding domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LT2_15_UPRpro_X2site

    Plasmid

    #194427

    Purpose

    Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    UPRpro
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LT2_32_PyruEth_X2site

    Plasmid

    #194428

    Purpose

    Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    PyruEth
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LT2_33_PyruPro_X2site

    Plasmid

    #194429

    Purpose

    Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    PyruPro
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LT2_34_EthPro_X2site

    Plasmid

    #194430

    Purpose

    Plasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    EthPro
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LT1_75_Donor_Tag_RPL13A-mTurq2

    Plasmid

    #194415

    Purpose

    Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    mTurquoise2 CDS
    

    Use

    Synthetic Biology; Used as donor dna in genome in…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG306-pTet07-leuYFP[CTT]-nLuc

    Plasmid

    #199757

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[CTT]

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    leuYFP[CTT]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG306-pTet07-leuYFP[CTC]-nLuc

    Plasmid

    #199758

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[CTC]

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    leuYFP[CTC]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

    Plasmid

    #199760

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[CGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

    Plasmid

    #199761

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_AGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[AGA]-cLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. C-SWAT 6FLAG

    Plasmid

    #182509

    Purpose

    C-SWAT compatible vector for C-terminal tagging with 6 FLAG

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    6 FLAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. C-SWAT IAA17-6FLAG (no NLS)

    Plasmid

    #193322

    Purpose

    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    IAA17
    

    Use

    Tags

    6FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. M2C5

    Plasmid

    #188606

    Purpose

    Minimal Conjugative Plasmid 2

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. M1C1

    Plasmid

    #188609

    Purpose

    Minimal Conjugative Plasmid 1

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. M4C16

    Plasmid

    #188610

    Purpose

    Minimal Conjugative Plasmid 4

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. M4C19

    Plasmid

    #188611

    Purpose

    Minimal Conjugative Plasmid 4

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p426-Cup1p-FusionRed

    Plasmid

    #188392

    Purpose

    Cu2+-dependent expression of red fluorescent protein in yeast cells

    Depositor

    Michihiko Kataoka

    Article

    Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

    Insert

    FusionRed
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1 promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pACT2-μ4

    Plasmid

    #198176

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-4 μ4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge