Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Yeast Expression

8,043 results

Showing: 5601 - 5640 of 8043 results

  9. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

    Enlarge

  11. P2061

    Plasmid
    #186299
    Purpose
    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10
    Inserts
    LexA-HBD-B112
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    2xLexop-minimalCyc1 and PACT1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186299-plasmid-map-sequence-id-367190

    Enlarge

  12. C-SWAT IAA17

    Plasmid
    #193319
    Purpose
    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    IAA17
    Use
    Tags
    IAA17
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193319-plasmid-map-sequence-id-383646

    Enlarge

  18. C-SWAT IAA17-3HA

    Plasmid
    #182517
    Purpose
    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    IAA17
    Use
    Tags
    3HA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182517-plasmid-map-sequence-id-366993

    Enlarge

  19. C-SWAT IAA17-3MYC

    Plasmid
    #182518
    Purpose
    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    IAA17
    Use
    Tags
    3MYC
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182518-plasmid-map-sequence-id-358261

    Enlarge

  28. C-SWAT GFP CL1 DAmP

    Plasmid
    #182513
    Purpose
    C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    CL1 and DAmP
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182513-plasmid-map-sequence-id-358273

    Enlarge

  34. pAG25-1199 cDNA

    Plasmid
    #160379
    Purpose
    N1199 narnavirus cDNA
    Depositor
    John McCusker
    Insert
    N1199
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    NA
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    160379-plasmid-map-sequence-id-314410

    Enlarge

  35. PJD1223

    Plasmid
    #160390
    Purpose
    Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT
    Depositor
    John McCusker
    Insert
    L-A Gag-Pol
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TRP1
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160390-plasmid-map-sequence-id-314529

    Enlarge

  36. N1199 launching

    Plasmid
    #160386
    Purpose
    Expression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequence
    Depositor
    John McCusker
    Insert
    N1199
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160386-plasmid-map-sequence-id-314521

    Enlarge

  37. pGAL1-VP1 (LEU2)

    Plasmid
    #182480
    Purpose
    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182480-plasmid-map-sequence-id-366129

    Enlarge

  38. deltaVP1d63C + VP2C-GFPdeg

    Plasmid
    #182481
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFPdeg
    deltaVP1d63C
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182481-plasmid-map-sequence-id-360432

    Enlarge

  39. mRuby3 only VLP

    Plasmid
    #182429
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182429-plasmid-map-sequence-id-360111

    Enlarge

  40. Linked mRuby3 VLP

    Plasmid
    #182430
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFP-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182430-plasmid-map-sequence-id-360112

    Enlarge

Showing: 5601 - 5640 of 8043 results