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pCfB4132

Plasmid

#106127

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYES2.1-cerMET14

Plasmid

#107441

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Publication

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET14 (MET14 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-3nmt1-mMaple3

Plasmid

#105167

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

kanMX6-3nmt1-mMaple3

Tags

3nmt1-mMaple3

Expression

Bacterial and Yeast

Promoter

3nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mYFP

Plasmid

#105144

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mYFP

Tags

81nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mYFP

Plasmid

#105141

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mYFP

Tags

41nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-3YFP-kanMX6

Plasmid

#105149

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3YFP-kanMX6

Tags

3YFP

Expression

Bacterial and Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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BB3rN_14*

Plasmid

#98550

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mEGFP

Plasmid

#105140

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P41nmt1-mEGFP

Tags

41nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-TC-OM45-FKBPx2

Plasmid

#104471

Purpose

Integrating plasmid for overexpression of OM45-FKBPx2.

Depositor

Benjamin Glick

Publication

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx2- HA

Expression

Yeast

Promoter

TPI1

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-TC-OM45-FKBPx4

Plasmid

#104472

Purpose

Integrating plasmid for overexpression of OM45-FKBPx4.

Depositor

Benjamin Glick

Publication

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx4- HA

Expression

Yeast

Promoter

TPI1

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-81nmt1

Plasmid

#105164

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-81nmt1

Tags

81nmt1

Expression

Bacterial and Yeast

Promoter

81nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-41nmt1

Plasmid

#105163

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-41nmt1

Tags

41nmt1

Expression

Bacterial and Yeast

Promoter

41nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-T|C-iGFP-PH(OSH1)

Plasmid

#105280

Purpose

Overexpresses iGFP-PH(Osh1) under the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

PH domain of Osh1 (SWH1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Promoter

TPI1

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-KEX2-iGFPx6

Plasmid

#105264

Purpose

Plasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Kex2 portion (KEX2 )

Tags

iGFPx6

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-iGFPx6

Plasmid

#105252

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL6-msGFPx3

Plasmid

#105254

Purpose

Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl6 portion (APL6 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRS406-GFP-SNC1(L96V)

Plasmid

#105278

Purpose

Integrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snc1 (SNC1 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Leucine 96 changed to Valine, intron removed

Promoter

TPI1

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE714

Plasmid

#103021

Purpose

expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHIS4-4.S (HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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