8,210 results
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Plasmid#20773DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only
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pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
ExpressionBacterial and YeastPromoterpADH1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPD 25QDProGFP p416
Plasmid#15568DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-H517Q-YFP
Plasmid#29620DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS316-GFP-COF1
Plasmid#31843DepositorAvailable SinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHB4
Plasmid#53462PurposeThis plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.DepositorInsertyeGFP-Snc1-Suc2
ExpressionYeastPromoterADHAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB305-K3L-vac-HA
Plasmid#20054DepositorInsertK3L
TagsHAExpressionYeastAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC820
Plasmid#20383DepositorInsertUAS cassette (FUS1)
ExpressionYeastMutationX=UbiM (see schematic)Available SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-DsRed
Plasmid#14366DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook2
Plasmid#198524PurposeExpression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook2 (HOOK2 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationHis488Gln substitutionPromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ECFP-ccdB
Plasmid#14299DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-ccdB-DsRed
Plasmid#14369DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCUP1-NMGR
Plasmid#1211DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-EYFP
Plasmid#14221DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis
Plasmid#60390PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKT-SCFP3A-HIS
Plasmid#64686PurposepKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.DepositorInsertSCFP3A
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
TagsL-IFP-F[2]ExpressionYeastPromoterGAL1Available SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
LD231
Plasmid#69193PurposeIntegration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg31-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LHP424-YCplac33-STE2-WT
Plasmid#32171DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only