7,943 results
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Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS134-Pleum
Plasmid#60339PurposeContains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS3-Pleum
Plasmid#60330PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS1-Pleum
Plasmid#59993PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS2-Pleum
Plasmid#59992PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
p414CYC_Hsp82_CycTER
Plasmid#61713Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterCYC1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_noTER
Plasmid#61714Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterTEF1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414ADH_Hsp82_noTER
Plasmid#61715Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterADH1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414CYC_Hsp82_noTER
Plasmid#61716Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterCYC1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C
Plasmid#60394PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptideDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C
Plasmid#60398PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB7 Cterminal tail
Plasmid#60399PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB8 Cterminal tail
Plasmid#60401PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail
Plasmid#60402PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C
Plasmid#60405PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p426 3-F GW
Plasmid#58556PurposepRS426 carrying Gateway cassette for three insertsDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB649
Plasmid#60136PurposeFirefly luciferase (Promega) driven by MET17 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-CU1-Pleum
Plasmid#60331PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNB689
Plasmid#60133PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB756
Plasmid#60149PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterLEU1prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB752
Plasmid#60155PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterADE17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB812
Plasmid#60160PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterRNR1prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ286
Plasmid#52227PurposeExpression of sgRNA precursor targeted against ade6L469DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
p41Nat 3-F GW
Plasmid#58553PurposeCEN vector with clonNat resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Skn7t-L-IFP-F[1] (Yeast)
Plasmid#52896PurposeSkn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.DepositorInsertSkn7t-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
Plasmid#52898PurposeYpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.DepositorInsertYpd*1-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationChanged Tryptophane 80 to AlaninePromoterGAL1Available sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pGLx2-set1-H1017K
Plasmid#53258PurposeGAL driven LexA-tagged SET1-catalytically dead (H1017K)DepositorInsertSET1
UseTagsLexAExpressionYeastMutationH1017K mutation - catalytically deadPromoterGAL1/10Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZHY566
Plasmid#50608PurposeTALEN expression vector for yeast, N152,C18 architectureDepositorTypeEmpty backboneUseTALENTagsExpressionYeastMutationPromoterTEFAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZHY565
Plasmid#50607PurposeTALEN expression vector for yeast, N152,C18 architectureDepositorTypeEmpty backboneUseTALENTagsExpressionYeastMutationPromoterTEFAvailable sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSS63
Plasmid#47951PurposertTA positive feedback loop with single tet operator in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEmerald-CaURA3
Plasmid#44890PurposeYeast tagging vector to create gene fusion with yeast optimized mEmerald with CaUra3 selectionDepositorInsertyomEmerald
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAH4
Plasmid#41828PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistanceDepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCM305
Plasmid#44550DepositorInsertHA-htz1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-PhiYellow-SpHis5
Plasmid#44651PurposeYeast tagging vector to create gene fusion with PhiYellow with SpHis5 selectionDepositorInsertphiYellow
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR-AmTrac-GS
Plasmid#47769Purposeexpresses AmTrac-GS in yeastDepositorInsertAmTrac-GS
UseTagsmcpGFPExpressionYeastMutationPromoterPMAAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFA12
Plasmid#46792PurposeTo express a version of Fun30 with a mutated CUE domain in budding yeastDepositorInsertFUN30, CUE domain mutated (FUN30 Budding Yeast)
UseTagsV5ExpressionYeastMutationFP82AAPromoterAvailable sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
UPR pBARUra3
Plasmid#45354DepositorInsertDHFR Ub Rek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
UPR pBAM Ura3
Plasmid#45355DepositorInsertDHFR Ub Mek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRM430
Plasmid#44530DepositorInsertHHT2
UseTagsExpressionYeastMutationhht2 deletion 4-30PromoterAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK611
Plasmid#44541DepositorInserthhf2
UseTagsExpressionYeastMutationdel (90-95)PromoterAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUK192
Plasmid#44962DepositorInsertLEU2
UseTagsExpressionYeastMutationHHTI-HHF1 is replaced with LEU2PromoterAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRM214
Plasmid#44527DepositorInsertHHT2
UseTagsExpressionYeastMutationK14RPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFX05
Plasmid#44531DepositorInsertHHT2
UseTagsExpressionYeastMutationK56QPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
UPR pBAL Ura3
Plasmid#45353DepositorInsertDHFR Ub Lek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1Available sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only