8,223 results

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pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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P413TEF-PYK1

Plasmid

#34605

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

TEF

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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FRP1432

Plasmid

#64509

Purpose

Expresses destabilized Citrine in yeast.

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

Citrine-ADH1-tail

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHLK

Plasmid

#64182

Purpose

harbours marker genes HIS3, LEU2 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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p414TEF_Hsp82_CycTER

Plasmid

#61711

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ACS1

Plasmid

#126712

Purpose

yeast plasmid complementing ACS1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acs1

Tags

6xHis,Myc

Expression

Yeast

Promoter

GAP

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Tags

HaloTag

Expression

Yeast

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ccdB

Plasmid

#188445

Purpose

Cloning Vector and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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HCP8

Plasmid

#166110

Purpose

This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5

Depositor

Matthias Heinemann

Insert

Whi5-sg106 (WHI5 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBKWH-Lam

Plasmid

#133899

Purpose

Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRS403-PGAL1-weakSC_GFP

Plasmid

#22315

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

Insert

GFP-rad9

Use

Integrating plasmid

Expression

Yeast

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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POM42-mCitrine

Plasmid

#69854

Purpose

N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046672

Plasmid

#153918

Purpose

Yeast expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pXP314

Plasmid

#26835

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-MYO1

Plasmid

#51570

Purpose

Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

MYO1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet17

Plasmid

#107453

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met17 (met17 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM394

Plasmid

#84204

Purpose

Disturb the expression of miR394 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR394

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 Bt δCOP

Plasmid

#73871

Purpose

Bos taurus Arcn1

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 Promoter- COPD

Expression

Yeast

Promoter

Ret2 Promoter

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pXRhMX

Plasmid

#63146

Purpose

HPH marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Article

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial and Yeast

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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