8,133 results

1. pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)

   Plasmid

   #179057

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early Golgi

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-SED5
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

   Plasmid

   #179056

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-SCS2tmh
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179055

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeleton

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-ARC35-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179053

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-SPC42-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

   Plasmid

   #178996

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046426

   Plasmid

   #149140

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGBW-m4046422

   Plasmid

   #145515

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pKT0211

   Plasmid

   #8734

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECitrine A206R
   

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with S65G, V68L, Q69M, S72A, T203Y, A206R

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-VPS16-iGFPx6

   Plasmid

   #105275

   Purpose

   Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Vps16 portion (VPS16 Budding Yeast)
   

   Tags

   iGFPx6

   Expression

   Yeast

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNMG

    Plasmid

    #1203

    Depositor

    Susan Lindquist

    Article

    True et al Nature 2004 Sep 9;431(7005):184-7. Epub

    Insert

    NM region of Sup35 (SUP35 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NM region (1-253aa) of Sup35

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB6679

    Plasmid

    #106158

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GTU-g

    Plasmid

    #81094

    Purpose

    Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-SKII-3XHA-fCLIP-Kan

    Plasmid

    #188923

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fCLIP-Kan
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAL FLAG72QDProCFPp303

    Plasmid

    #15586

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q Delta Pro (HTT Human)
    

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pAV0586

    Plasmid

    #133473

    Purpose

    Integration into fission yeast genome at lys3 locus with bsdMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. YN2_1_LT84_RPL13A

    Plasmid

    #177712

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS416 Gal-RNQ1

    Plasmid

    #18684

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pDRF1-GW mScarlet-T2A-mTurquoise2

    Plasmid

    #118431

    Purpose

    Produces equimolar expression of mScarlet and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    mScarlet-T2A-mTurquoise2
    

    Expression

    Yeast

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJW1515

    Plasmid

    #62385

    Purpose

    pFA6a Osm1-yoEGFP::LEU2

    Depositor

    Jonathan Weissman

    Article

    Williams et al Science. 2014 Nov 7;346(6210):748-51. do

    Insert

    OSM1-EGFP fusion
    

    Tags

    EGFP

    Expression

    Yeast

    Promoter

    Adh1p

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. ZJOM12

    Plasmid

    #133638

    Purpose

    Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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