8,086 results

1. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

   Plasmid

   #184407

   Purpose

   yeast surface display of the SARS-CoV-2 Delta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   L452R+T478K

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEDJ437

   Plasmid

   #177270

   Purpose

   USER cloning-compatible 2μ vector (HIS3)

   Depositor

   Michael Jensen

   Article

   Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAB42

   Plasmid

   #17268

   Depositor

   Stuart Schreiber

   Article

   Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

   Type

   Empty backbone

   Use

   Tags

   B42•HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pJT131_GalL_cCDA1-NGBE3

   Plasmid

   #145095

   Purpose

   Expressing base editor cCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-NGBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH646-2µ-RLuc

   Plasmid

   #40600

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   Renilla Luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046619

   Plasmid

   #149218

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. U6-U57C

   Plasmid

   #169928

   Purpose

   WT U6 with a U57C mutation

   Depositor

   Aaron Hoskins

   Article

   van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

   Insert

   U6 U57C
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p41Neo MagicFluor

   Plasmid

   #58564

   Purpose

   p41Neo carrying both mating type markers buffered by the Cyc1 terminator

   Depositor

   Leonid Kruglyak

   Insert

   Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM2_12

   Plasmid

   #183061

   Purpose

   Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

   Depositor

   Yvonne Nygård

   Article

   Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

   Inserts

   mCherry
   BM3R1-HAA1-mTurquoise2
   

   Use

   Synthetic Biology

   Tags

   mCherry and mTurquoise2

   Expression

   Yeast

   Mutation

   Promoter

   HAA1 and Synthetic promoter

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMM2_4

    Plasmid

    #183057

    Purpose

    HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mRuby2
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mRuby2 and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and YGP1

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMM2_10

    Plasmid

    #183059

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM2_9

    Plasmid

    #183058

    Purpose

    Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMM2_11

    Plasmid

    #183060

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. deltaVP1 + VP2C-GFP

    Plasmid

    #166676

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LD102

    Plasmid

    #69161

    Purpose

    Integration of GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg2-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046219

    Plasmid

    #153926

    Purpose

    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMZ377

    Plasmid

    #74214

    Purpose

    Combined sgRNA/Cas9 vector with LEU2 marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPTK016-3-RFP_Intra

    Plasmid

    #84975

    Purpose

    Type 3, Red fluorescent protein

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Red fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (1677 c to g and 1794 a to g)

    Promoter

    Non

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046217

    Plasmid

    #149071

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. S3_002_026_096

    Plasmid

    #134403

    Purpose

    pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    None and none

    Expression

    Yeast

    Mutation

    None and none

    Promoter

    pGPM1 and pRPL18B

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge