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pJLI-SUP35 (delta)2-5

Plasmid

#1138

Depositor

Susan Lindquist

Publication

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted, but …

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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TM104b(WT)

Plasmid

#1159

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
sup35-R320I

Plasmid

#1199

Depositor

Susan Lindquist

Publication

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed arg 320 to isoleucine

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Publication

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pBK1

Plasmid

#1213

Depositor

Susan Lindquist

Publication

Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

Insert

Hsc82::LEU2 (HSC82 Budding Yeast)

Use

Integration

Expression

Yeast

Mutation

hsc82::LEU2 deletion select for plasmid using LE…

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(WT)

Plasmid

#1162

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(K351N)

Plasmid

#1163

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

K351N

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(WT)

Plasmid

#1165

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(K351N)

Plasmid

#1166

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Mutation

K351N

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(E368Q)

Plasmid

#1167

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

E368Q

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Available since

Sept. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Available since

Sept. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Available since

Sept. 18, 2026

Availability

Academic Institutions and Nonprofits only

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Leu Cen/ars Tef mNg-MinDec, Tef MinEec-mRuby2

Plasmid

#252613

Purpose

Cen/ars plasmid for expressing mNg-MinD and MinEec-mRuby2 in budding yeast under Leu2 auxotrophic selection

Depositor

Scott Coyle

Publication

Bolshakov et al ACS Synth Biol. 2025 Dec 16. doi: 10.102

Inserts

mNeongreen-MinDec fusion
MinEec-mRuby2

Tags

mNeongreen and mRuby2

Expression

Yeast

Available since

Aug. 10, 2026

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-URA3

Plasmid

#122911

Purpose

LYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 31, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pcdc2short-MCP-NLS-tdSG

Plasmid

#255013

Purpose

expression of MCP-NLS-tandemStayGold from medium-strength P.cdc2 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.cdc2(355-1,AfeImut)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Mutation

1 nucleotide removed in cdc2 promoter to abolish …

Promoter

Spom\P.cdc2

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Plon1-MCP-NLS-tdSG

Plasmid

#255010

Purpose

expression of MCP-NLS-tandemStayGold from weak P.lon1 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.lon1(451-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.lon1

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-NLS-tdSG

Plasmid

#255011

Purpose

expression of MCP-NLS-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Ppak1-MCP-NLS-tdSG

Plasmid

#255012

Purpose

expression of MCP-NLS-tandemStayGold from weak P.pak1 (P.shk1) promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.pak1(630-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.pak1

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-2xNLS-NES-tdSG

Plasmid

#255014

Purpose

expression of MCP-2xNLS-NES-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-2xNLS-NES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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