8,266 results
-
Plasmid#90253Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 113 amino acids and amino acid N110…AvailabilityAcademic Institutions and Nonprofits only -
pSL7 - Brr2 del247 N1104L
Plasmid#90257Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 247 amino acids and N1104LAvailabilityAcademic Institutions and Nonprofits only -
pSL11 - Brr2 del422 N1104L
Plasmid#90261Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid N110…AvailabilityAcademic Institutions and Nonprofits only -
pSL12 - Brr2 del422 Q904E
Plasmid#90262Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid Q904EAvailabilityAcademic Institutions and Nonprofits only -
pSL13 - Brr2 del422 R1107L
Plasmid#90263Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid R110…AvailabilityAcademic Institutions and Nonprofits only -
pHES942
Plasmid#90408PurposeExpresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-SIC1
ExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
Sub2-3 in pRS314
Plasmid#90454PurposeyeastDepositorInsertSUB2
ExpressionYeastMutationsub2-3 mutationAvailabilityAcademic Institutions and Nonprofits only -
Sub2-4 in pRS314
Plasmid#90455PurposeyeastDepositorInsertSUB2
ExpressionYeastMutationsub2-4 mutationAvailabilityAcademic Institutions and Nonprofits only -
Sub2-6 in pRS314
Plasmid#90456PurposeyeastDepositorInsertSUB2
ExpressionYeastMutationsub2-6 mutationAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4G
Plasmid#104502PurposeUsed to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter geneDepositorInsertScPGM2 promoter-yEGFP
ExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4K
Plasmid#104506PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSpGAL1 promoter-yEGFP
ExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4O
Plasmid#104510PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSpdGAL2 promoter-yEGFP
ExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4P
Plasmid#104511PurposeUsed to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSptGAL2 promoter-yEGFP
ExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3.loxP
Plasmid#63200PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K3.loxP
Plasmid#63202PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K6.loxP
Plasmid#63203PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5.loxP
Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO5.loxP
Plasmid#63206PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
L284F Cus2p
Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pHES936
Plasmid#89199PurposeExpresses DIG1 under control of GAL1 promoterDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1860N
Plasmid#90966Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1862D
Plasmid#90967Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 T1865K
Plasmid#90968Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 A1871E
Plasmid#90969Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 T1872E
Plasmid#90970Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 T1861P
Plasmid#90971Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1862Y
Plasmid#90972Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 H1863E
Plasmid#90973Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 K1864E
Plasmid#90974Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 N1869D
Plasmid#90975Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1870N
Plasmid#90976Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 I1875T
Plasmid#90977Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
brr2-Q904E pRS313
Plasmid#111413Purposebrr2-Q904E (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationQ904E aa mutationAvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107A pRS313
Plasmid#111415Purposebrr2-R1107A (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationR1107A aa mutationAvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107M pRS313
Plasmid#111417Purposebrr2-R1107M (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationR1107M aa mutation (note, not an RP allele)AvailabilityAcademic Institutions and Nonprofits only -
pV1338
Plasmid#111443PurposeSolo vector pV1326 + sgScADE2DepositorInsertCaCas9/sgScADE2
UseCRISPRExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
yFusionRed
Plasmid#111916Purposeyeast optimized FusionRedDepositorInsertyFusionRed
ExpressionYeastPromoterGPDAvailabilityAcademic Institutions and Nonprofits only -
pJW1669
Plasmid#112043PurposeCEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URADepositorInsertNSG2
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1672
Plasmid#112046PurposeCEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URADepositorInsertPST1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1674
Plasmid#112048PurposeCEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URADepositorInsertSWP1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pEMY01AB-PRO
Plasmid#127714PurposeIntegrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tadh1
Plasmid#127723PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPact1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Ppre3-ITE-Teno1
Plasmid#127724PurposeYeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPpre3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tdpp1
Plasmid#127725PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPact1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Phta1-ITE-Tvma2
Plasmid#127726PurposeYeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPhta1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPrps3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPeft2AvailabilityAcademic Institutions and Nonprofits only