8,051 results

1. pGBW-m4046333

   Plasmid

   #145544

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046262

   Plasmid

   #145524

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046525

   Plasmid

   #145523

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046290

   Plasmid

   #145522

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046185

   Plasmid

   #145508

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046416

   Plasmid

   #145388

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046570

   Plasmid

   #145558

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046466

   Plasmid

   #145466

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046498

   Plasmid

   #145503

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 leader protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 leader protein (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046218

    Plasmid

    #145498

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046588

    Plasmid

    #145465

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pRS426-ECM_unstable

    Plasmid

    #127607

    Purpose

    high-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    Unstable ECM33 intron (Mut)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS416-PTEF1-MCP-3P-ECM33_intron

    Plasmid

    #127601

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 3′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS416-PTEF1-MCP-5M-ECM33_intron

    Plasmid

    #127600

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416-PTEF1-MCP-5P-ECM33_intron

    Plasmid

    #127599

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS416-PTEF1-MCP-ECM33_intron

    Plasmid

    #127598

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS416-PTEF1-MCP-no_intron

    Plasmid

    #127597

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    1xFLAG-MCP expression cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIplac204-pOst1-ESCargo(Orange)

    Plasmid

    #140157

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YIplac128-pOst1-ESCargo(Orange)

    Plasmid

    #140158

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIplac128-pOst1-ESCargo(Crimson)

    Plasmid

    #140156

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

    Plasmid

    #140155

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YIplac204-pOst1-ESCargo(Crimson)

    Plasmid

    #140154

    Purpose

    Expresses a vacuolar cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ADH1pro_GCR2_SIR4

    Plasmid

    #138619

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    GCR2 (GCR2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ADH1pro_GCR1_SIR4

    Plasmid

    #138618

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    GCR1 (GCR1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ADH1pro_TYE7_SIR4

    Plasmid

    #138617

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    TYE7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ADH1pro_CBF1_SIR4

    Plasmid

    #138616

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    CBF1 (CBF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS416-ECM33_intron

    Plasmid

    #127596

    Purpose

    low-copy URA-selectable plasmid, ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS416-RPL27B_intron

    Plasmid

    #127604

    Purpose

    low-copy URA-selectable plasmid, RPL27B intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    RPL27B intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS416-APS3_intron

    Plasmid

    #127605

    Purpose

    low-copy URA-selectable plasmid, APS3 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    APS3 intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRS416-HNT2_intron

    Plasmid

    #127603

    Purpose

    low-copy URA-selectable plasmid, HNT2 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    HNT2 intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p415:iCas9

    Plasmid

    #138266

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Cas9-D10A, H840A

    Promoter

    GalL

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS424-ysg(G:H)

    Plasmid

    #138257

    Purpose

    Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    GFP targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS424-ysg(NT)

    Plasmid

    #138258

    Purpose

    Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSB1C3-ysg(NT)

    Plasmid

    #138263

    Purpose

    Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMV23

    Plasmid

    #128887

    Purpose

    Position 4 module- STOP (stop codon)

    Depositor

    Gilles Charvin

    Article

    Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

    Insert

    STOP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMV16

    Plasmid

    #128894

    Purpose

    Position 6 module- LEU2

    Depositor

    Gilles Charvin

    Article

    Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMV47

    Plasmid

    #128866

    Purpose

    Position 1 module- IX301997 (yeast integrative region IX301997)

    Depositor

    Gilles Charvin

    Article

    Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

    Insert

    Yeast integrative region chrIX301997
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMV22

    Plasmid

    #128884

    Purpose

    Position 3 module- Venus

    Depositor

    Gilles Charvin

    Article

    Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMV42

    Plasmid

    #128888

    Purpose

    Position 4 module- mCherry-STOP

    Depositor

    Gilles Charvin

    Article

    Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

    Insert

    mCherry+STOP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. S3_007_026_MV1

    Plasmid

    #134419

    Purpose

    pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge