8,430 results
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Plasmid#194639PurposeEmpty pAGE2.0 plasmid with pBAD inducible promoterDepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
ExpressionYeastPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C3
Plasmid#189620PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRemoved repeat region, 10-kbp deletion, and minor…Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneTagsGal4 Activation domainExpressionYeastPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2
Plasmid#196826PurposeYeast two-hybrid ; Gal4 DNA binding domainDepositorTypeEmpty backboneTagsGal4 DNA binding domainExpressionYeastPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
UG75-ADH1-CDS1-3HA
Plasmid#198596PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM4
Plasmid#198414PurposeExpress mAID-Nb(LaM4) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM4)
ExpressionYeastPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM8
Plasmid#198415PurposeExpresesses mAID-Nb(LaM8) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM8)
ExpressionYeastPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
ExpressionYeastPromoterTetO7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_IL50_HOlocus
Plasmid#194426PurposeExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiaeDepositorInsertCas9
ExpressionYeastMutationNAAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C2
Plasmid#141247PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2DepositorPublicationInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…Available sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
ExpressionYeastPromoterCUP1 promoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FUS-FusionRed
Plasmid#188393PurposeCu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cellsDepositorAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook2
Plasmid#198524PurposeExpression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook2 (HOOK2 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationHis488Gln substitutionPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-DGK1-3HA
Plasmid#198595PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-PAH1-3HA
Plasmid#198597PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ1A
Plasmid#197381PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-1 σ1A
TagsGAL4-DNA binding domain fragment, HA tag, and SV4…ExpressionYeastMutationContains an extra 42 nucleotides encoding 14 resi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-αC
Plasmid#197650PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-2 αC
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationSer677T substitutionPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3A
Plasmid#198174PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitution in codon 225PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3B
Plasmid#198175PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3B
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only