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8,432 results

Showing: 3481 - 3500 of 8432 results

  1. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  2. pGADT7-αC

    Plasmid
    #197650
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-2 αC
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Ser677T substitution
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197650-plasmid-map-sequence-id-388876

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  3. pACT2-μ3A

    Plasmid
    #198174
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3A
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitution in codon 225
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198174-plasmid-map-sequence-id-387537

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  4. pACT2-μ3B

    Plasmid
    #198175
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3B
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198175-plasmid-map-sequence-id-387536

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  9. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Publication
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
    Tags
    EGFP
    Expression
    Yeast
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  17. pAG25-1199 cDNA

    Plasmid
    #160379
    Purpose
    N1199 narnavirus cDNA
    Depositor
    John McCusker
    Insert
    N1199
    Expression
    Yeast
    Promoter
    NA
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    160379-plasmid-map-sequence-id-314410

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  18. pESC-NAT-Ago-Dcr

    Plasmid
    #160381
    Purpose
    Overexpression of Ago-Dcr fusion protein in yeast
    Depositor
    John McCusker
    Insert
    Ago- Dcr
    Expression
    Yeast
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160381-plasmid-map-sequence-id-314500

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  19. N1199 launching

    Plasmid
    #160386
    Purpose
    Expression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequence
    Depositor
    John McCusker
    Insert
    N1199
    Expression
    Yeast
    Promoter
    PGK1
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160386-plasmid-map-sequence-id-314521

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  20. Linked Free NES (M)

    Plasmid
    #182436
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    FPPS(M)-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182436-plasmid-map-sequence-id-360120

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Showing: 3481 - 3500 of 8432 results