7,941 results

1. pMEL17

   Plasmid

   #107923

   Purpose

   TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDN-T2dMFot

   Plasmid

   #44558

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

   Inserts

   PTETREG promoter
   rtetR-M2::FFF
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

   Promoter

   PTETREG

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

   Plasmid

   #79614

   Purpose

   Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Inserts

   SpCas9
   PmCDA1
   

   Use

   Tags

   SH3 domain and SHL

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1 and pGal10

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG416GAL-ccdB-mRuby3

   Plasmid

   #166139

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG416GAL-ccdB-EGFP

   Plasmid

   #14195

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB6638

   Plasmid

   #106165

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS423-mitoT

   Plasmid

   #174525

   Purpose

   Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

   Depositor

   Oleh Khalimonchuk

   Article

   Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

   Insert

   Yeast mitochondrial intermembrane tether
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL669

   Plasmid

   #169148

   Purpose

   Encodes OptoQ-AMP2

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAL722

   Plasmid

   #169149

   Purpose

   Encodes OptoQ-AMP3

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   A79Q

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL716

    Plasmid

    #169150

    Purpose

    Encodes OptoQ-AMP4

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge