-
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1
Plasmid#58431PurposeExpresses LexA-ER-B42 in yeast cellsDepositorInsertLexA-ER-B42
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB
Plasmid#14148DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
LT1_33_pTEFmut8-mCherry
Plasmid#177706PurposeConstruct for genome integration expressing constitutively mCherry under pTEFmut8 promoterDepositorInsertpTEFmut8-mCherry
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAS9id
Plasmid#141251PurposeGalactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML107
Plasmid#67639PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformationDepositorInsertsCas9
single guide RNA expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterpSNR52 and pTDH3 (aka GAP promoter)Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXN22-Dest
Plasmid#105085PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubG-3xHAExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB
Plasmid#14156DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS415-GPD
Plasmid#183940PurposeControl vector that has a GPD promoter with no insertDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
UseTagsExpressionBacterial and YeastMutationE366G (See depositor comment below)PromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only