7,941 results

1. pFTK004

   Plasmid

   #171276

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PgpdA ANIA_08041 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYES2.1-cerMET6

   Plasmid

   #107438

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET6 (MET6 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GAL FLAG25QDProCFPp303

   Plasmid

   #15584

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Use

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. D771 leu2::LYS2 Disruptor Converter

   Plasmid

   #51677

   Purpose

   Yeast marker swap plasmid for converting LEU2 to LYS2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   LYS2 (LYS2 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS416-Gal4-dCas9-VP64

   Plasmid

   #71128

   Purpose

   This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

   Depositor

   Ronald Davis

   Article

   Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

   Insert

   Gal4-dCas9-VP64
   

   Use

   Tags

   NLS n-terminal, N terminal Gal4 Activator domain,…

   Expression

   Yeast

   Mutation

   Promoter

   Tef1

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-ytdTomato-CaURA3

   Plasmid

   #118461

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRFLIP42

   Plasmid

   #65518

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-Cit-attR1 and attR2-mCer-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRFLIP35

   Plasmid

   #65513

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-AFPt9-attR1 and attR2-TFPt9-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRFLIP32

   Plasmid

   #65511

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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