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pDR-AmTrac1;2

Plasmid

#47765

Purpose

expresses AmTrac1;2 in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
M34 Xdsh PDZ bait-pGBT9

Plasmid

#17190

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Xdsh (dvl2.L Frog)

Use

Tags

Gal4-DBD

Expression

Yeast

Mutation

Dsh bp 343-1191

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-LS

Plasmid

#47767

Purpose

expresses AmTrac-LS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-LS

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pAG424GPD-ccdB-DsRed

Plasmid

#14368

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pRS316-Cwc25

Plasmid

#111343

Purpose

Cwc25 in pRS316(Ura) vector

Depositor

Christine Guthrie

Insert

CWC25

Use

Tags

none

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pTH751-CEN-RLuc/min53maxCFLuc

Plasmid

#38217

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first 53 codons of the original FLuc gene wer…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pAG415-ADH1p-HA-rap1-K9R

Plasmid

#84531

Purpose

This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

ADH1

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG424GAL-ccdB-ECFP

Plasmid

#14175

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-HA-TMSec66-SrtA (pKS105)

Plasmid

#50033

Purpose

Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

SrtA(strep)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSL10 - Brr2 del422 WT

Plasmid

#90260

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ECFP-ccdB

Plasmid

#14294

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ccdB-EYFP

Plasmid

#14222

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pNC861

Plasmid

#20391

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP-reporter

Use

Tags

Expression

Yeast

Mutation

X=UbiY (see schematic) linker: ek

Promoter

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 A. victoria, Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Promoter

Available since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pAG303GPD-EYFP-ccdB

Plasmid

#14326

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG305GPD-EYFP-ccdB

Plasmid

#14330

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge