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pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

Plasmid

#126739

Purpose

yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2, acs1*, ald6

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C and A30S, S629N (please see depositor comm…

Promoter

GAP

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des1

Plasmid

#110212

Purpose

Yeast Surface Display of G6des1

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des1

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA151

Plasmid

#128526

Purpose

Expresses mutant Ber e 1 in Pichia

Depositor

Marcos Alcocer

Insert

mutant Ber e 1

Expression

Yeast

Promoter

AOX1

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-CPR

Plasmid

#126742

Purpose

yeast plasmid overexpressing CPR

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

cpr

Tags

6xHis

Expression

Yeast

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-eGFP

Plasmid

#126720

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Tags

6xHis,Myc

Expression

Yeast

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-LacI-Mit1AD

Plasmid

#126722

Purpose

LacI-Mit1AD on pGAPZ_ A expression vector

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

GAP

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUC_delta ald1

Plasmid

#126708

Purpose

vector for knocking out ald1 in Komagataella phaffii

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

HIS4

Expression

Yeast

Promoter

His

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ACS1

Plasmid

#126712

Purpose

yeast plasmid complementing ACS1 from Komagataella phaffii

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acs1

Tags

6xHis,Myc

Expression

Yeast

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yosfGFP-T2A-mCherry

Plasmid

#118448

Purpose

Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yosfGFP-T2A-mCherry

Expression

Yeast

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eGFP-T2A-mCherry

Plasmid

#118422

Purpose

Produces equimolar expression of eGFP and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eGFP-T2A-mCherry

Expression

Yeast

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB4613

Plasmid

#126752

Purpose

Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

Depositor

Michael Jensen

Publication

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

CDC14 (CDC14 Budding Yeast)

Use

Integrative plasmid

Expression

Yeast

Promoter

pTRX2_5xUAS

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMST-1312

Plasmid

#118075

Purpose

pTPI1-cexA-tCYC1

Depositor

Michael Sauer

Publication

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTPI1-cexA-tCYC1

Expression

Yeast

Promoter

pTPI1

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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wt Cus2p

Plasmid

#73046

Purpose

Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

Depositor

Aaron Hoskins

Publication

Rodgers et al RNA. 2015 Dec 2.

Insert

CUS2p (CUS2 Budding Yeast)

Tags

6x His tag fused to TEV cleavage site

Expression

Bacterial and Yeast

Promoter

T7

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1837

Plasmid

#123058

Purpose

_-factor pheromone

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

_-factor [3-4a]

Expression

Yeast

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Publication

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(406)

Plasmid

#122068

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Publication

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pWS939

Plasmid

#123051

Purpose

Pheromone responsive transcription factor

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste12 [3]

Expression

Yeast

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWS627

Plasmid

#123064

Purpose

G___ bicistronic protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste4-2A-Ste18 [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS788

Plasmid

#123059

Purpose

_-factor protease

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Bar1 [3]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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