8,388 results

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pJK273

Plasmid

#71563

Purpose

Produces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid desaturase (form 2)

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDS1U-PKS12

Plasmid

#70150

Purpose

Fusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

PKS12 (PKS12 Fusarium graminearum)

Expression

Yeast

Promoter

CUP1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP42

Plasmid

#65518

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-mCer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP35

Plasmid

#65513

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-TFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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BS-Lys2

Plasmid

#69199

Purpose

complementation of yeast auxotrophic marker LYS2

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

LYS2

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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LD121

Plasmid

#69172

Purpose

Integration of GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg21-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD111

Plasmid

#69165

Purpose

Integration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg11-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD106

Plasmid

#69163

Purpose

Integration of GFP tag at Atg6 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg6-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

Plasmid

#68274

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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LD117

Plasmid

#69169

Purpose

Integration of GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg17-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRG227

Plasmid

#64532

Purpose

Episomal E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP32

Plasmid

#65511

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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PSUC2-GFPantisense-CYC1t-pRS413

Plasmid

#64404

Purpose

SUC2 regulated expression of an antisense RNAi construct for GFP

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

GFPantisense

Expression

Yeast

Promoter

SUC2

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP3

Plasmid

#65451

Purpose

pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP reporter

Expression

Yeast

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pITGFP3

Plasmid

#65452

Purpose

pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP-CLN2PEST reporter

Expression

Yeast

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c6gRNA_RPR1t

Plasmid

#64384

Purpose

encodes c6 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c6 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_a1gRNA_RPR1t

Plasmid

#64378

Purpose

encodes a1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

a1 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pYC16

Plasmid

#63913

Purpose

Recipient overexpressing vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

CFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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