7,942 results

1. pCYC1pr-GNSI (LEU)

   Plasmid

   #111450

   Purpose

   GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

   Depositor

   Alexey Merz

   Article

   Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

   Inserts

   CYC1 Promoter (CYC1 Budding Yeast)
   Nyv1 Cytosolic Domain (aa 6-230)
   Snc1 Transmembrane Domain (TMD)
   SUC2 CDS
   SUC2 terminator
   

   Use

   Tags

   mGFP5

   Expression

   Yeast

   Mutation

   M109I (Naturally occurring SNP) and The first 21 …

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GAL 72Q+ProGFPp416

   Plasmid

   #15582

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 72Q + Pro (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pCfB8380

   Plasmid

   #126914

   Purpose

   Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtE
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB9337 (pgRNA_IV-1_NatMX)

   Plasmid

   #161590

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK3F

   Plasmid

   #85770

   Purpose

   N-ICE plasmid pK3F

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-KanMX-loxP-3FLAG
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDBD-R

   Plasmid

   #50571

   Purpose

   Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572

   Depositor

   Alexander Varshavsky

   Article

   Turner et al Science. 2000 Sep 22;289(5487):2117-20.

   Insert

   DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   M1R (B-gal)

   Promoter

   Gal1

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pGPD-luxAB(HIS)

   Plasmid

   #1106

   Depositor

   Susan Lindquist

   Article

   Parsell et al Nature 1994 Dec 1;372(6505):475-8.

   Insert

   bacterial luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   n/a

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pKT0140

   Plasmid

   #8732

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECitrine
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with S65G, V68L, Q69M, S72A, T203Y

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJB170

   Plasmid

   #158600

   Purpose

   Transcription factors ADR1c, PIP2c, OAF1c

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Inserts

   ADR1c
   PIP2c
   OAF1c
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Coexpressed Free GFP-NES

    Plasmid

    #182491

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    GFP-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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