8,418 results
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Plasmid#68274PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherryDepositorInsertSte7DS NLS mCherry
TagsmCherryExpressionYeastPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD117
Plasmid#69169PurposeIntegration of GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg17-EGFP
TagsEGFPExpressionYeastAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG227
Plasmid#64532PurposeEpisomal E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
PSUC2-GFPantisense-CYC1t-pRS413
Plasmid#64404PurposeSUC2 regulated expression of an antisense RNAi construct for GFPDepositorInsertGFPantisense
ExpressionYeastPromoterSUC2Available sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneTagsyEGFP reporterExpressionYeastAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneTagsyEGFP-CLN2PEST reporterExpressionYeastAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
3x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64391Purposeencodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorPublicationInsertyeast enhanced BFP2
ExpressionYeastAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB6 Cterminal tail
Plasmid#60403PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c6gRNA_RPR1t
Plasmid#64384Purposeencodes c6 gRNADepositorPublicationInsertc6 gRNA
ExpressionYeastAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_a1gRNA_RPR1t
Plasmid#64378Purposeencodes a1 gRNADepositorPublicationInserta1 gRNA
ExpressionYeastAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC16
Plasmid#63913PurposeRecipient overexpressing vector to construct CFP tagged fusionsDepositorTypeEmpty backboneTagsCFPExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1508
Plasmid#62362PurposepFA6a BirA-mVenus-SSH1::HIS5DepositorInsertBirA-mVenus-Ssh1
TagsBirA-mVenusExpressionYeastPromoterGal1Available sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1516
Plasmid#62386PurposepFA6a Osm1(M1A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
TagsEGFPExpressionYeastMutationMethionine 1 to AlaninePromoterAdh1pAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB761
Plasmid#60156PurposeFirefly luciferase (Promega) driven by ADE17 promoter.DepositorInsertFLuc
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterADE17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only