7,943 results

1. pRS426-ACT_stable

   Plasmid

   #127609

   Purpose

   high-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   Stable ACT1 intron (Mut)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKT0103

   Plasmid

   #8718

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yEVenus
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS414 Hsh155 K410N

   Plasmid

   #87280

   Purpose

   Encodes Hsh155 K410N for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   K410N

   Promoter

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNA0304

   Plasmid

   #165612

   Purpose

   Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   NotI site replaced the CAN1 spacer in parent vect…

   Promoter

   SNR52

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYC52

   Plasmid

   #63908

   Purpose

   Recipient integrative vector to construct CFP tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. GTH-r

   Plasmid

   #81106

   Purpose

   Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   mCherry
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS416 Gal TDP43 Q331K

   Plasmid

   #27461

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Glutamine 331 mutated to Lysine (Q331K)

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pRS405 PEX14-VAC8-RFP

   Plasmid

   #166844

   Purpose

   Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, PEX14 Budding Yeast)
   

   Use

   Tags

   PEX14 transmembrane domain and RFP

   Expression

   Yeast

   Mutation

   PEX14 transmembrane domain

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS405 SEC66-VAC8-RFP

   Plasmid

   #166843

   Purpose

   Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, SEC66 Budding Yeast)
   

   Use

   Tags

   RFP and SEC66 transmembrane domain

   Expression

   Yeast

   Mutation

   SEC66 transmembrane domain

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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