8,057 results

1. pMG343

   Plasmid

   #176183

   Purpose

   preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   TPK2+ccdB dropout cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRR-AR

   Plasmid

   #73045

   Purpose

   Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

   Depositor

   David McMillen

   Article

   Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

   Insert

   Androgen receptor (AR Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   please see depositor comments below

   Promoter

   glyceraldehyde phosphate dehydrogenase (GPD)

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW1661

   Plasmid

   #112035

   Purpose

   OsTIR1 expression cassette marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   TIR1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRG205MX

   Plasmid

   #64535

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ymScarlet

   Plasmid

   #111917

   Purpose

   yeast optimized mScarlet

   Depositor

   Patrick Lajoie

   Article

   Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

   Insert

   ymScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG206MX

   Plasmid

   #64536

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHLUM (version 2)

   Plasmid

   #64166

   Purpose

   harbours marker genes HIS3, LEU2, URA3 and MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH650-RLuc/staFLuc

   Plasmid

   #29696

   Depositor

   Tobias von der Haar

   Article

   Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

   Insert

   Firefly Luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTA-HUH

    Plasmid

    #221152

    Purpose

    HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Insert

    HisG-URA3-HisG
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRO117

    Plasmid

    #233823

    Purpose

    MoClo-YTK; type3; Reporter; mNeonGreen

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT253

    Plasmid

    #40943

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG2J

    Plasmid

    #102882

    Purpose

    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Inserts

    OsTIR1
    mGFP-AID
    

    Use

    Tags

    AID(71-114)

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and TEF1

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYES2/25Q

    Plasmid

    #1384

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (25Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. JME4599

    Plasmid

    #129660

    Purpose

    NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBF3038

    Plasmid

    #26850

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDR FLIP43 GPS1

    Plasmid

    #101084

    Purpose

    GA biosensor expression plasmid

    Depositor

    Alexander Jones, Wolf Frommer

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    GPS1
    

    Use

    Tags

    6HIS and cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB2197

    Plasmid

    #67551

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge