7,943 results

1. pROS13

   Plasmid

   #107927

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJAS1668

   Plasmid

   #158593

   Purpose

   VioE-ePTS1 CEN6/ARS4

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   VioE-ePTS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. M3499 ura3::ADE2 Disruptor Converter

   Plasmid

   #51674

   Purpose

   Yeast marker swap plasmid for converting URA3 to ADE2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   ADE2 (ADE2 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADE2

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG306GPD-EGFP-ccdB

   Plasmid

   #14308

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-PATagRFP-KanMX6

   Plasmid

   #87027

   Purpose

   Tag S. pombe genes in C terminal with PATagRFP

   Depositor

   Julien Berro

   Insert

   PATagRFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR-GW FLIPglu-2µΔ13V

   Plasmid

   #28000

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-2µΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   H152A

   Promoter

   Available since

   June 10, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCfB2804

   Plasmid

   #63644

   Purpose

   pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYEXC3GH

   Plasmid

   #49027

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC6-TEFkFLP(LYS2)

   Plasmid

   #49458

   Purpose

   overespression of Flp step-arrest mutant in yeast, LYS2 selection

   Depositor

   Marc Gartenberg

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   TEF1

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIV494

    Plasmid

    #177701

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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