8,057 results

1. pRS306-URA3-PHIS3-PfV-GS-Sapphire

   Plasmid

   #116932

   Purpose

   Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   HIS3

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ZJOM18

   Plasmid

   #133631

   Purpose

   Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-NAB2-GFP (NAB2 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p406GAL1

   Plasmid

   #17437

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GAL1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-GFP(S65T)-His3MX6

   Plasmid

   #41598

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRL_GI_PCB28

   Plasmid

   #98745

   Purpose

   For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

   Inserts

   heme oxygenase 1 (TED4 Mustard Weed)
   Phycocyanobilin:ferredoxin oxidoreductase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   ADH1m and PGK1

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Gal:mCherry-uTEV3

   Plasmid

   #135453

   Purpose

   Yeast expression of uTEV3 fused to mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-uTEv3
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   S219V mutation improves stability , I138T/S153N/T…

   Promoter

   Gal

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUDP082

   Plasmid

   #103875

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gRNA-trp-HYB

   Plasmid

   #64331

   Purpose

   trp1 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of trp1 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-link-yomEos2-Kan

   Plasmid

   #44944

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomEos2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS423-GPD-wrmScarlet1-10-CYC1 terminator

    Plasmid

    #158584

    Purpose

    Expresses wrmScarlet1-10 in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGI3EM18

    Plasmid

    #135484

    Purpose

    Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunH2Bpr hph-tdTomato ADH1t
    

    Use

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2Bpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

    Plasmid

    #173452

    Purpose

    Contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

    Insert

    mCherry-sfGFP-ADH1ter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_MFE1_hrGFP

    Plasmid

    #84617

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCYC1m_yeGFP

    Plasmid

    #64389

    Purpose

    encodes yeast enhanced GFP under control of minimal pCYC1 promoter

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    yeast enhanced GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pCYC1

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG426GAL-ccdB

    Plasmid

    #14155

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pILGFPB5A

    Plasmid

    #83556

    Purpose

    Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    GAL1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-mEOS3.2-KanMX6

    Plasmid

    #87030

    Purpose

    Tag S. pombe genes in C terminal with mEOS3.2

    Depositor

    Thomas Pollard, Julien Berro

    Article

    Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    mEOS3.2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSFS2A-CaKan

    Plasmid

    #189565

    Purpose

    Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

    Depositor

    Richard Bennett

    Article

    Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

    Insert

    CaKan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG303-Gal-PR50

    Plasmid

    #84905

    Purpose

    induce expression of dipeptide repeat PR in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    PR50 (C9orf72 Synthetic)
    

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS306-OsTIR1F74A

    Plasmid

    #198416

    Purpose

    Expresses OsTIR1F74A under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    OsTIR1F74A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge