8,128 results

1. pAF9D94 (pAF10C6)

   Plasmid

   #185848

   Purpose

   Expressing artificial trans-repressor in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PHAC1>TetR>CYC8>PABF1
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIT6EG7mlh

   Plasmid

   #185887

   Purpose

   In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (23 copy).

   Depositor

   Claudia Vickers

   Insert

   see comments
   

   Expression

   Yeast

   Mutation

   ERG20 F96W N127W

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIPKAH

   Plasmid

   #185869

   Purpose

   Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIALD2HMGr

   Plasmid

   #185867

   Purpose

   Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIALD2E

   Plasmid

   #185866

   Purpose

   Knocking out ALD6

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD(-125, 40)- ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJCble

   Plasmid

   #185861

   Purpose

   The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAF4E9D

   Plasmid

   #185844

   Purpose

   Expressing artificial trans-activator in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PHAC1>LacI>TUP1>PABF1
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAF9C3

   Plasmid

   #185843

   Purpose

   Expressing artificial trans-activator in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PHAC1>RevTetR_r1.7>SIN3C>PABF1
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAF9D91

   Plasmid

   #185845

   Purpose

   Expressing artificial trans-activator in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PHAC1>TetR>SIN3C>PABF1
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIT7A6

    Plasmid

    #185896

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pILAC2

    Plasmid

    #185889

    Purpose

    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Expression

    Yeast

    Mutation

    ERG20 F96C; Xd.CRtYB E83K

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIT6EG7m

    Plasmid

    #185885

    Purpose

    A backbone plasmid for in vivo gene amplification (HamAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pINER4R

    Plasmid

    #185884

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pILGFP5EG3

    Plasmid

    #185878

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pILGFP4BG6

    Plasmid

    #185877

    Purpose

    A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pILGFP3AE4

    Plasmid

    #185873

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pILGFP3A5C

    Plasmid

    #185872

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pILGFP1DFB

    Plasmid

    #185871

    Purpose

    A parental plasmid for in vivo gene amplification at RPL25 locus

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIALD2S

    Plasmid

    #185865

    Purpose

    Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCKan

    Plasmid

    #185862

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker

    Depositor

    Claudia Vickers

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge