8,133 results

1. pRS416-PTEF1-MCP-3P-ECM33_intron

   Plasmid

   #127601

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins inserted near 3′ end of intron
   

   Expression

   Yeast

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS416-PTEF1-MCP-5M-ECM33_intron

   Plasmid

   #127600

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
   

   Expression

   Yeast

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS416-PTEF1-MCP-5P-ECM33_intron

   Plasmid

   #127599

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins inserted near 5′ end of intron
   

   Expression

   Yeast

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS416-PTEF1-MCP-ECM33_intron

   Plasmid

   #127598

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron
   

   Expression

   Yeast

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YIplac204-pOst1-ESCargo(Orange)

   Plasmid

   #140157

   Purpose

   Expresses a regulatable secretory cargo for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
   

   Expression

   Yeast

   Promoter

   TPI1

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YIplac128-pOst1-ESCargo(Orange)

   Plasmid

   #140158

   Purpose

   Expresses a regulatable secretory cargo for yeast at the LEU2 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
   

   Expression

   Yeast

   Promoter

   TPI1

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIplac128-pOst1-ESCargo(Crimson)

   Plasmid

   #140156

   Purpose

   Expresses a regulatable secretory cargo for yeast at the LEU2 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
   

   Expression

   Yeast

   Promoter

   TPI1

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

   Plasmid

   #140155

   Purpose

   Expresses a regulatable secretory cargo for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
   

   Expression

   Yeast

   Promoter

   TPI1

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIplac204-pOst1-ESCargo(Crimson)

   Plasmid

   #140154

   Purpose

   Expresses a vacuolar cargo for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

   Insert

   pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL
   

   Expression

   Yeast

   Promoter

   TPI1

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ADH1pro_GCR2_SIR4

    Plasmid

    #138619

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    GCR2 (GCR2 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ADH1pro_GCR1_SIR4

    Plasmid

    #138618

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    GCR1 (GCR1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ADH1pro_TYE7_SIR4

    Plasmid

    #138617

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    TYE7
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ADH1pro_CBF1_SIR4

    Plasmid

    #138616

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    CBF1 (CBF1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS416-ECM33_intron

    Plasmid

    #127596

    Purpose

    low-copy URA-selectable plasmid, ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron
    

    Expression

    Yeast

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416-RPL27B_intron

    Plasmid

    #127604

    Purpose

    low-copy URA-selectable plasmid, RPL27B intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    RPL27B intron
    

    Expression

    Yeast

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS416-APS3_intron

    Plasmid

    #127605

    Purpose

    low-copy URA-selectable plasmid, APS3 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    APS3 intron
    

    Expression

    Yeast

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS416-HNT2_intron

    Plasmid

    #127603

    Purpose

    low-copy URA-selectable plasmid, HNT2 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    HNT2 intron
    

    Expression

    Yeast

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p415:iCas9

    Plasmid

    #138266

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Cas9-D10A, H840A

    Promoter

    GalL

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS424-ysg(G:H)

    Plasmid

    #138257

    Purpose

    Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    GFP targeting sgRNAs G and H
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS424-ysg(NT)

    Plasmid

    #138258

    Purpose

    Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge