8,436 results
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Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
TagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEOS3.2-KanMX6
Plasmid#87030PurposeTag S. pombe genes in C terminal with mEOS3.2DepositorInsertmEOS3.2
ExpressionBacterial and YeastAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfB3052(gRNA X-4, XI-3, XII-5)
Plasmid#73294PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
ExpressionYeastPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRR-AR
Plasmid#73045PurposeConstitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)DepositorInsertAndrogen receptor (AR Human)
ExpressionYeastMutationplease see depositor comments belowPromoterglyceraldehyde phosphate dehydrogenase (GPD)Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3-GFP-Atg8
Plasmid#69194PurposeSingle copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertGFP-Atg8
TagsEGFPExpressionYeastAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRG206
Plasmid#64527PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIYC04
Plasmid#64741PurposeYeast expression plasmidDepositorTypeEmpty backboneExpressionYeastAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYC56
Plasmid#63910PurposeRecipient integrative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-mRFP-ura4MX6
Plasmid#49183Purposegenomic mRFP tagging in S. pombeDepositorTypeEmpty backboneTagsmRFPExpressionYeastAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-12Pk-kanMX6
Plasmid#49176Purposegenomic 12Pk epitope tagging in S. pombeDepositorTypeEmpty backboneTags12PkExpressionYeastAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXP842
Plasmid#46068DepositorTypeEmpty backboneExpressionYeastPromoterADH2Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-CaURA3
Plasmid#44881PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selectionDepositorInsertyomKO2
ExpressionYeastAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-SpHis5
Plasmid#44849PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selectionDepositorInsertyomEos2
ExpressionYeastAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPA-mCherry-SpHis5
Plasmid#44855PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selectionDepositorInsertyoPA-mCherry
ExpressionYeastAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECerulean-CaUra3
Plasmid#44648DepositorInsertyECerulean
ExpressionYeastAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRMH122-LexA-CRY2PHR
Plasmid#40613PurposeExpresses LexA-CRY2PHR for light-inducible protein expression when coupled with VP16-CIBDepositorPublicationInsertLexA-CRY2PHR
ExpressionYeastPromoterADH1Available sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH727-CEN-RLuc/staCFLuc
Plasmid#38211DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by